Abstract: Objective To explore the effect and possible molecular mechanism of Hedyotis diffusa on the proliferation, migration and invasion of bladder cancer cells. Methods U-19-19 cells were divided into control group, Hedyotis diffusa group, miR-NC group, miR-485-5p group, anti-miR-NC group, anti-miR-485-5p group, Hedyotis diffusa +anti-miR-NC group, Hedyotis diffusa+anti-miR-485-5p group, Hedyotis diffusa+anti-miR-485-5p+LY294002 group. Cell counting kit (CCK-8), Transwell experiment, and real-time fluorescent quantitative PCR (RT-qPCR) were used to detect cell proliferation, migration, invasion and miR-485-5p expression. Western blot was performed to detect the phosphorylation levels of phosphatidylinositol-3-hydroxykinase (PI3K) and protein kinase B (AKT). Results After the intervention of Hedyotis diffusa, the proliferation rate, and the cell numbers of migrat and invade of KU-19-19 cells were significantly reduced, and the expression of miR-485-5p was significantly increased (P＜0.05). After overexpression of miR-485-5p, the proliferation rate, and the cell numbers of migrat and invade of KU-19-19 cells were significantly reduced (P＜0.05). After inhibiting the expression of miR-485-5p, the proliferation rate, and the cell numbers of migrat and invade of KU-19-19 cells were significantly increased (P＜0.05). Inhibition of miR-485-5p could reverse the inhibitory effect of Hedyotis diffusa on KU-19-19 cell proliferation, migration and invasion (P＜0.05). LY294002 could reverse the promoted effect of inhibiting miR-485-5p on proliferation, migration and invasion of KU-19-19 cells treated with Hedyotis diffusa (P＜0.05). Conclusions Hedyotis diffusa can inhibit the proliferation, migration and invasion of bladder cancer cells by up-regulating miR-485-5p. The mechanism may be related to the inhibition of PI3K/AKT signaling pathway.

Key words: Hedyotis diffusa, miR-485-5p, bladder cancer, proliferation, migration and invasion, PI3K/AKT signaling pathway