Etomidate alleviates LPS-induced rat myocardial cell line H9C2 injury by upregulating miR-290-5p

Abstract

Abstract: Objective To investigate the protective mechanism of etomidate (Eto) on lipopolysaccharide (LPS) induced myocardial injury. Methods H9C2 cells were divided into control (Co) group, LPS group (1 μg/mL LPS treatment for 6 h), Eto+LPS group, miR-con+LPS group, miR-290-5p+LPS group, Eto+anti-miR-con+LPS group, and Eto+anti-miR-290-5p+ LPS group. Tetramethylazozolium blue (MTT) method and flow cytometry were used to detect cell viability and apoptosis; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect miR-290-5p expression. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in cell culture fluid. Results Compared to the control group, the expression of miR-290-5p in H9C2 cells in the LPS group reduced (P＜0.05), the cell survival rate reduced(P＜0.05), the apoptosis rate increased (P＜0.05), and the level of TNF-α and IL-6 significantly increased (P＜0.05); compared with the LPS group, the expression of miR-290-5p in H9C2 cells in the Eto+LPS group was increased (P＜0.05), and the cell survival rate was decreased (P＜0.05), apoptotic rate was decreased (P＜0.05), TNF-α and IL-6 content were decreased (P＜0.05); compared with the miR-con+LPS group, the survival rate of H9C2 cells in the miR-290-5p+LPS group increased (P＜0.05), the apoptosis rate reduced (P＜0.05), the level of TNF-α and IL-6 reduced (P＜0.05); when compared with the Eto+anti-miR-con+LPS group, the survival rate of H9C2 cells in the Eto+anti-miR-290-5p+LPS group decreased(P＜0.05), and apoptosis rate increased (P＜0.05), the level of TNF-α and IL-6 increased(P＜0.05). Conclusions Etomidate reduces LPS-induced myocardial cell apoptosis and inflammatory response by up-regulating miR-290-5p.

Key words: etomidate, lipopolysaccharide, miR-290-5p, cardiomyocytes injury, apoptosis

CLC Number:

R542.2

LEI Jia-xiu, ZHU Ya-ping, ZHANG Yu. Etomidate alleviates LPS-induced rat myocardial cell line H9C2 injury by upregulating miR-290-5p[J]. Basic & Clinical Medicine, 2021, 41(6): 848-853.

References

[1] 霍江, 李强, 于颖群, 等. 丙泊酚和依托咪酯对脓毒症小鼠炎症反应与氧化应激的影响[J]. 临床麻醉学杂志, 2015, 31: 1221-1223.
[2] 全宗宗, 罗海鸣. 依托咪酯联合富氢液对脓毒症小鼠氧化应激、炎症损伤情况及血清HMGB1、MDA水平的影响分析[J]. 遵义医学院学报, 2019, 42:291-294.
[3] 潘宁. 依托咪酯对脓毒症大鼠全身炎症反应及肾上腺的影响[D]. 大连:大连医科大学, 2014, 1-32.
[4] Zheng G, Qu H, Li F, et al. Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway[J]. Braz J Med Biol Res, 2018, 51: e7655.doi: 10.1590/1414-431X20187655.
[5] 李萌芳, 陈隆望, 胡系意, 等. 黄芩苷对脂多糖诱导的H9C2心肌细胞炎症的保护作用及机制研究[J]. 中华急诊医学杂志, 2019, 28: 983-988.
[6] 卿城, 黄舒颖, 黄自坤, 等. 人参皂苷Rg1对LPS致心肌细胞损伤的保护作用及其对细胞lincRNA-COX2表达的影响[J]. 广东医学, 2017, 38: 2436-2438.
[7] 李异, 王慷慨, 蒋永芳, 等. 心型脂肪酸结合蛋白对脂多糖所致心肌细胞损伤的保护作用[J]. 中南大学学报, 2015, 45: 7-13.
[8] 张洋, 焦皓, 王红军, 等. 5种静脉麻醉药对成年大鼠心肌细胞的影响[J]. 徐州医学院学报, 2013, 33: 813-814.
[9] 周婷, 尤纱纱, 李旋, 等. 舒芬太尼复合依托咪酯预处理对大鼠心肌缺血-再灌注损伤的保护作用[J]. 江苏医药, 2015, 41: 1625-1627.
[10] 吴敏, 庞宏宣, 张文斌, 等. 依托咪酯对体外循环直视心脏手术患者炎症因子的影响[J]. 实用医学杂志, 2011, 27: 102-104.
[11] 张月顺, 南锡浩, 芦相玉, 等. 依托咪脂对LPS诱导的神经胶质细胞中(IL)-1β表达的影响[J]. 中外医疗, 2019, 38: 13-15.
[12] Xiang JF, Yu JC, Zhu JY. Up-regulation of miR-27 extenuates lipopolysaccharide-induced injury in H9C2 cells via modulating ICAM1 expression[J]. Genes Genomics, 2019, 41: 1467-1474.
[13] An R, Feng J, Xi C, et al. miR-146a attenuates sepsis-induced myocardial dysfunction by suppressing IRAK1 and TRAF6 via targeting ErbB4 expression[J]. Oxid Med Cell Longev, 2018, 2018: 7163057.doi: 10.1155/2018/7163057.
[14] 王惠. microRNA-21-3p通过靶基因SORBS2调控LPS诱导的脓毒症心肌病[D]. 南京:南京医科大学, 2016, 1-111.
[15] Li Y, Liu X, Du A, et al. miR-203 accelerates apoptosis and inflammation induced by LPS via targeting NFIL3 in cardiomyocytes[J]. J Cell Biochem, 2019, 120: 6605-6613.