Abstract: Objective To investigate the effect of SRPX2 protein on migration and polarization of human monocytes THP-1 derived macrophages. Methods PMA was used to induce macrophages derived from human THP-1 cells. Transwell , immunoflurescence and Western blot were used to determine the cell immigration, signal protein level and location of SRPX2 and uPAR respectively. After SRPX2 was added. IFN-γand LPS was used to induce M1 polarization cells, RT-PCR was used to measure M1/M2 markers. Results The migration of THP-1 derived macrophages were significantly increased by SRPX2 (p<0.01) and antagonized by uPAR neutralization antibody (p<0.01). SRPX2 markedly promoted the expression of CD206/IL-6, while decreased the expression of CD40/IL-6 (both p<0.01). The colocalization of SRPX2/uPAR/CD11b was confirmed. The phosphorylation of FAK and Akt was activated by SRPX2. Conclusions SRPX2 may promote the migration and M2 polarization of human monocytes THP-1 originated macrophage via uPAR/CD11b/FAK/Akt pathway.

Key words: SRPX2, macrophages, migration, polarization