Abstract: Objective To investigate the effect of capsaicin and cisplatin on the proliferation and immunogenic cell death of human osteosarcoma cells. Methods MTT assay was used to examine the growth inhibiting effects of capsacin and cisplation on MG-63 cells; Mitochondrial membrane potential (MMP) was used to investigate the apoptosis; flow cytometry was used to detect the expression of calreticulin(CRT) on the cell membrane, fluorescein enzymatic method was used to detect the release of ATP and ELISA was used to detect the secretion of high mobility group B1 (HMGB1). Results Capsaicin and cisplatin can inhibit MG-63 cells proliferation in the dose-dependent manner and induce MG-63 apoptosis（P＜0.01）. Only capsaicin can induce translocation of CRT from endoplasmic reticulum to the cell surface and release of extracellular ATP and HMGB1（P＜0.01）. Conclusions Capsaicin can induce human osteosarcoma cells apoptosis and immunogenic cell death.

Key words: capsaicin, immunogenic cell death, osteosarcoma