Abstract: Objective Explore the expression of ADAM15、MMP-2 and MMP-9 in different breast cancer cell lines through experiment to investigate the relativity of the metastasis of breast cancer cell.Methods Transwell chamber experiments were used to test the metastasis of two breast cancer cell lines.Immunocytochemical were used to carry out qualitative analysis,localization and semi-quantitative detection and quantitative detection through Western blot for ADAM15、MMP-2 and MMP-9.Results The migration of MDA-MB-231 breast cancer cell line is stronger than the MCF-7,s.The expression of ADAM15、MMP-2 and MMP-9 in the MDA-MB-231 breast cancer cell line are higher than that in the MCF-7(immunohistochemistry：P＜0.001，P＜0.05，P＜0.001；Western blot：P＜0.01，P＜0.05，P＜0.01)，and the difference of ADAM15 is more obvious than MMP-9 and MMP2 in two cell lines .Conclusion The expression of ADAM15、MMP-2 and MMP-9 closely correlate to the metastasis of breast cancer cells,and infer that ADAM15 may have a closer relationship to the metastasis of breast cancer cells than the others.

Key words: ADAM15 , MMP-2 , MMP-9 , breast cancer cell, metastasis