Abstract: Objective The thesis aims to observe and evaluate the effect of hypoxia on CacyBP/SIP nuclear translocation of the cell lines of human colon carcinoma. Methods taking cDNA sequence of colon carcinoma cell line HCT-116 cells as the subject, the study mainly builds overexpressed lentiviral vector with Green fluorescent protein CacyBP/SIP by Molecular cloning, and then transfects it into SW480 cells of colon carcinoma. Taking CoCl2 as the Stimulating factor, it tests the location and expression of the CacyBP/SIP with Laser Scanning Confocal Microscope and Western blot. Results the construction of lentiviral vectors for the overexpression of CacyBP / SIP, was transfected into colon cancer cells successfully, and it has been found that before the stimulation of CoCl2, CacyBP/SIP is located and expressed in the cytoplasm, and both in cytoplasm and nucleus after the stimulation of CoCl2. Conclusion The stimulation of CoCl2 can lead to the nuclear translocation of CacyBP/SIP.

Key words: CacyBP/SIP, CoCl2, nuclear translocation, colon cancer