Abstract: Objective To observe the effects of Oleanolic acid on islet cell proliferation and apoptosis under high glucose and fat toxicity. Methods Mouse primary islet cell was cultivated under high glucose and fat ,then different concentration of Oleanolic acid are administrated for intervention, radioimmunoassay to detect insulin secretion , islet cell proliferative activity was determined by MTT assay; islet cell apoptosis was determined by flow cytometry . Results Islet cell proliferation and insulin secretion were inhibited by the mixed culture medium of glucose and palmitic acid, Oleanolic acid can against it. Conclusion Oleanolic acid can protect islet cell from high glucolipotoxicity, enhance viability of islet cell and inhibit islet cell apoptosis.

Key words: Key words: Oleanolic acid , Glucolipide toxicity, Cell proliferation , Cell apoptosis