Upregulation of miR-1287-5p promotes paclitaxel resistance in human esophageal cancer cell line KYSE30

doi:10.16352/j.issn.1001-6325.2025.11.1473

Basic & Clinical Medicine ›› 2025, Vol. 45 ›› Issue (11): 1473-1479.doi: 10.16352/j.issn.1001-6325.2025.11.1473

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Upregulation of miR-1287-5p promotes paclitaxel resistance in human esophageal cancer cell line KYSE30

WANG Juzheng¹, LU Jiayu², WANG Qingshi¹, LIU Wen², BAO Bo², LI Yiming², LU Qiang^3*

1. Department of Thoracic Surgery, Xianyang First People's Hospital, Xianyang 712000;
2. College of Basic Medicine, Air Force Medical University, Xi'an 710032;
3. Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University, Xi'an 710038, China

Received:2024-10-24 Revised:2025-01-08 Online:2025-11-05 Published:2025-10-24
Contact: ^*luqiang0103o@163.com

Abstract

Abstract: Objective To discover the mechanism by which miR-1287-5p affects the sensitivity of human esophageal cancer cell line KYSE30 to paclitaxel. Methods KYSE30 cells were divided into control group, group of transfected with miR-1287-5p mimic and inhibitor groups. RT-qPCR was used to detect the transfection rate. A paclitaxel-resistant cell line was established and cell viability and half inhibitory concentration (IC₅₀) were detected by CCK-8 assay. The scratch test, Transwell chamber test and TUNEL method were used to evaluate the migration, invasion and apoptosis of cells . Western blot and immunocytochemistry (ICC) were used to detect the expression and localization of Akt and GSK-3β in cells. Results The expression level of miR-1287-5p in the miR-1287-5p mimic group was significantly higher than that in the miR-NC group and control group (P＜0.05), while the expression level of the miR-1287-5p inhibitor group was significantly lower than that in miR-NC group and control group (P＜0.05). The cell survival rate of the miR-1287-5p mimic group was higher than that of the miR-NC group and the miR-1287-5p inhibitor group (P＜0.05). The cell migration rate and number of invasive cells in the miR-1287-5p mimic group were higher than those of the miR-NC group(P＜0.05), and the apoptosis rate was lower than that of the miR-NC group (P＜0.05). While in miR-1287-5p, the cell migration rate was lower and invasive cells were less in the inhibitor group and the apoptosis rate was increased as compared to the miR-NC group (P＜0.05).The expression of Akt and GSK-3β in the miR-1287-5p mimic group was significantly increased than in the miR-NC group (P＜0.05), while the expression in the miR-1287-5p inhibitor group was significantly inhibited than in miR-NC group (P＜0.05). Conclusions Up-regulation of miR-1287-5p can promote the migration and invasion of paclitaxel-resistant cell lines, inhibit cell apoptosis and thereby promote the development of paclitaxel resistance.

Key words: miR-1287-5p, esophageal cancer, paclitaxel resistance

CLC Number:

R735.1

WANG Juzheng, LU Jiayu, WANG Qingshi, LIU Wen, BAO Bo, LI Yiming, LU Qiang. Upregulation of miR-1287-5p promotes paclitaxel resistance in human esophageal cancer cell line KYSE30[J]. Basic & Clinical Medicine, 2025, 45(11): 1473-1479.

References

[1] 吴佳林,张若兰,刘燕群,等.Hsa_circ_0140180通过调控miR-1287-5p影响食管鳞状细胞癌细胞的迁移及侵袭能力[J].中国肿瘤生物治疗杂志, 2023, 30:114-122.
[2] 陈君,李林艳,赵晓勇,等.罗哌卡因通过AKT/GSK-3β/β-catenin信号通路对膀胱癌细胞生长和化疗效果影响[J].中华肿瘤防治杂志, 2023:1208-1222.
[3] Chang Y, Jia HQ, Xu B, et al. Metadherin inhibits chemosensitivity of triple-negative breast cancer to paclitaxel via activation of AKT/GSK-3β signaling pathway[J]. Chem Biol Drug Des, 2024,103:14416. doi: 10.1111/cbdd.14416.
[4] Jin M, Yuan T, Tian K, et al. Oncogenic circ-SLC16A1 promotes progression of non-small cell lung cancer via regulation of the miR-1287-5p/profilin 2 axis[J]. Cell Mol Biol Lett, 2024,29:43. doi: 10.1186/s11658-024-00549-x.
[5] Xiao Y, Gu S, Yao W, et al. Circ_0047921 acts as the sponge of miR-1287-5p to stimulate lung cancer progression by regulating proliferation, migration, invasion, and glycolysis of lung cancer cells[J]. World J Surg Oncol, 2022,20:108. doi:10.1186/s12957-021-02466-1.
[6] Han Q, Ma L, Shao L, et al. Circ_0075804 regulates the expression of LASP1 by targeting miR-1287-5p and thus affects the biological process of retinoblastoma[J]. Curr Eye Res, 2022, 47:1077-1086.doi: 10.1080/02713683.2022.2053164.
[7] Guo W, Wu Z, Chen J, et al. Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immun-otherapy by promoting ferroptosis[J]. J Immunother Cancer, 2022, 10:e004381. doi: 10.1136/jitc-2021-004381.
[8] 赵磊,王一斐,薛国勇.lncRNA BLACAT1通过靶向miR-374b-5p对卵巢癌细胞紫杉醇耐药性的影响及其机制[J].中国老年学杂志, 2022, 42:5616-5621.
[9] 史铁伟,李天柱,周静,等.5-脂氧合酶激活蛋白抑制剂MK886对人食管癌细胞系增殖和凋亡的影响[J].基础医学与临床,2022,42:56-61.
[10] 张洋,赵辰戈,程荔春,等.miR-152-3p表达下调降低紫杉醇耐药人卵巢癌细胞A2780T对紫杉醇的耐药性[J].中国药理学与毒理学杂志, 2024, 38:22-30.
[11] Hu W, Shen J, Tao Y, et al. CCDC85C suppresses colorectal cancer cells proliferation and metastasis through activating GSK-3β and promoting β-catenin degradation[J]. Cell Signal, 2023,109:110799. doi: 10.1016/j.cellsig.2023.110799.
[12] Wang N, Hou SY, Qi X, et al. LncRNA LPAL2/miR-1287-5p/EGFR axis modulates TED-derived orbital fibroblast activation through cell adhesion factors[J]. J Clin Endocrinol Metab, 2021,106:2866-2886. doi: 10.1210/clinem/dgab256.

Upregulation of miR-1287-5p promotes paclitaxel resistance in human esophageal cancer cell line KYSE30

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[3]	CHEN Jia-yang, YANG Yu-fei, YI Dan-hui, FENG Li. Analysis and prognosis of advanced esophageal cancer treated by Traditional Chinese Medicine [J]. Basic & Clinical Medicine, 2020, 40(2): 182-186.
[4]	. MBP-1 overexpression affects the proliferation and apoptosis of esophageal cancer cell Eca-109 [J]. Basic & Clinical Medicine, 2014, 34(2): 246-248.
[5]	Hui GUO; Jian-bing DING; Wei SUN; Tong ZHANG. Research Progress Of Related Genes In Xinjiang Kazakh's Esophageal Cancer [J]. Basic & Clinical Medicine, 2010, 30(4): 428-430.