Effects of lidocaine on mitophagy in lung tissues of rat models with acute lung injury

doi:10.16352/j.issn.1001-6325.2023.09.1394

Abstract

Abstract: Objective To investigate the effect of lidocaine on mitophagy in model rat of acute lung injury(ALI). Methods The rats were divided into the control group, acute lung injury group (group ALI:intraperitoneal injection of 10 mg/kg of lipopolysaccharide(LPS) and lidocaine intervention group (group lidocaine:after intraperitoneal injection of LPS,receiving a loading dose of lidocaine of 10 mg/kg and continued for 3 h with dosage of 10 mg/kg per hour). The rats were sacrificed 24 hrs after LPS, blood was collected for blood gas analysis, and calculated for oxygenation index (OI).Thoracotomy was performed immediately after blood collection, the samples of left lung tissue were collected to determine the expression of mitophagy-related protein E3 ubiquitin ligase (Parkin), microtubule-associated protein 1 light chain 3 (LC3) and p62 by Western blot, and then LC3-Ⅱ/LC3-Ⅰ was calculated. After-wards, right-upper lung tissue structure was microscopy and the mitochondrial autophagy in the right lower lung tissue was examined by transmission electron microscopy. Results Compared with control group, the level of Parkin, LC3-Ⅱ/LC3-Ⅰ and PaCO₂ was increased in both group ALI and group lidocaine(P＜0.05),while PaO₂,OI,pH and p62 were decreased(P＜0.05). Compared with group ALI,the level of Parkin and LC3-Ⅱ/LC3-Ⅰ was decreased in group of lidocaine intervention (P＜0.05),while PaO₂,OI,pH and p62 were increased(P＜0.05).The results of light microscopy showed intact alveolar structure and normal cell morphology in group control, inflammatory cell infiltration, interval thickening and alveolar collapse were found in group ALI, and less degree in group lidocaine than in group ALI. Transmission electron microscopy showed that the lung tissue and mitochondrial structure were normal in group control; in group ALI, the lung tissue cell was found to be swollen and formed with physalides companying by mitochondrial autophagosomes; The degree of injury in group lidocaine was decreased as compared with group ALI. Conclusions Lidocaine attenuates acute lung injury and the mechanism may be related to inhibition of mitophagy hyperactivation.

Key words: lidocaine, acute lung injury, mitochondria, autophagosome

CLC Number:

R614

XU Guiping, CHEN Xueying, ZHANG Yuxuan, Malipate·YILIAIKEBAIER. Effects of lidocaine on mitophagy in lung tissues of rat models with acute lung injury[J]. Basic & Clinical Medicine, 2023, 43(9): 1394-1398.

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