Snail1在糖尿病大鼠肾组织中表达增加

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (9): 957-963.

Snail1在糖尿病大鼠肾组织中表达增加

方开云娄晶磊肖瑛石明隽桂华珍郭兵张国忠

贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室贵阳医学院病理生理学教研室

收稿日期:2007-11-23 修回日期:2007-12-25 出版日期:2008-09-25 发布日期:2008-09-25
通讯作者: 张国忠

Snail1 expression increased in the renal tissues of diabetic rats

Kai-yun FANG, Jing-lei LOU, Ying XIAO, Ming-juan SHI, Hua-zhen GUI, Bing GUO, Guo-zhong ZHANG

Guiyang Medical College Guiyang Medical College

Received:2007-11-23 Revised:2007-12-25 Online:2008-09-25 Published:2008-09-25
Contact: Guo-zhong ZHANG

摘要/Abstract

摘要： 目的观察Snail1在糖尿病大鼠肾组织中的表达，并初步探讨其在糖尿病肾病（DN）发生、发展中的作用。方法大鼠随机分为对照组（C）、糖尿病组（DM）、胰岛素治疗组（A）。检测Snail1、α-平滑肌肌动蛋白（α-SMA）、E-钙黏素及纤连蛋白（FN）的蛋白和（或）mRNA表达。结果Snail1阳性染色见于各组DM大鼠肾小管。从16周开始在DM大鼠肾小管上皮细胞可见α-SMA蛋白阳性表达。肾皮质Snail1与FN蛋白和mRNA的表达水平均显著增加（P＜0.05），而治疗组上述指标显著降低（P＜0.05）。E-cadherin蛋白及mRNA表达与Snail1呈负相关。结论 Snail1基因和蛋白在DM大鼠肾小管高表达，并可能通过介导FN生成和抑制E-cadherin转录触发肾小管上皮细胞EMT参与了DN的发生和发展。

关键词: Snail1, E-钙黏素, α-平滑肌肌动蛋白, 糖尿病肾病, 大鼠

Abstract: Objective To explore the expression of snail1 in renal tissues of diabetic rats ,and to investigate its contribution to the progression of diabetic nephropathy.Methods Rats were randomly divided into control groups,DM and insulin-treated groups . The protein and(or) mRNA expression of Snail1，E-cadherin, α-SMA and FN in renal tissue were detected. Results The Snail1 protein strongly positive stainings were observed in renal tubules of DM rats. In DM rats α-SMA tubules staining were seen from 16th week. The proteins and mRNA of Snail1 and FN were significantly up-regulated in renal cortex of diabetic rats（P＜0.05）, while down-regulated in the insulin-treated diabetic rats （P＜0.05）. The expression of E-cadherin protein and mRNA were contrary to the snail1.Conclusion These findings suggest that snail1 expression is up-regulated in the renal tubules of rats with DM , which may involve in the pathogenesis of diabetic nephropathy by inducing FN and tubular epithelial-mesenchymal transition.

Key words: Snail1, E-cadherin, α-SMA, Diabetic nephropathy, rat

方开云娄晶磊肖瑛石明隽桂华珍郭兵张国忠. Snail1在糖尿病大鼠肾组织中表达增加[J]. 基础医学与临床, 2008, 28(9): 957-963.

Kai-yun FANG; Jing-lei LOU; Ying XIAO; Ming-juan SHI; Hua-zhen GUI; Bing GUO; Guo-zhong ZHANG. Snail1 expression increased in the renal tissues of diabetic rats[J]. Basic & Clinical Medicine, 2008, 28(9): 957-963.

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