关键词: 左旋丁基苯酞, l-NBP, 胶质细胞, NF-κB, IκB-α, 炎症反应

Abstract: Objective：To observe the effect of l-3-n-butylphthalide（l-NBP）on the protein expression of nuclear factor-κB and IκB-α in primary cultured mouse brain glial cells following oxygen-glucose deprivation/ reoxygenation（OGD/R）. Methods：Cultured mouse brain glial cells in vitro and established OGD/R model. Glial cells were divided into five groups: normal control（NC）group, OGD24h/R 24h group，l-NBP 20μmol/L group，l-NBP 100μmol/L group，l-NBP 500μmol/L group. The protein expression of NF-κB in nuclear and IκB-α in endochylema were analyzed by Western blot. Results：Comparing with NC group, OGD24h/R 24h group had a significantly higher expression of NF-κB (P<0.01）. The expression of NF-κB in l-NBP 100μmol/L group and l-NBP 500μmol/L group were evidently lower than that in OGD24h/R 24h group（P<0.01）. The degradation of IκB-α in OGD24h/R 24h group was significantly higher than that in NC group（P<0.01）. It was evident that the degradation of IκB-α in l-NBP 500μmol/L group was lower than that in OGD24h/R 24h group（P<0.05）. Conclusions：l-NBP can inhibit secondary inflammation of glial cells following OGD/R by decreasing the degradation of IκB-α and inhibitting activation of NF-κB.

Key words: l-3-n-butylphthalide, l-NBP, glial cell, nuclear factor-κB, IκB-α, inflammatory reaction