关键词: 精浆中性a一1,4一糖苷酶, 透明质酸酶, 男性不育

Abstract: Objective To study the relationship between neutral alpha—1,4 glycosidase(α-1,4-G )activity of human seminal plasma and the positive rate and activity intensity of hyaluronidase(HYD) .Method 1.To collect 38 infertile men’s semen, who were between 24 and 40 years old and be devided into 2 groups according to the result of neutral α-1,4-G activity test. They were normal neutral α-1,4-G activity group(26 cases) and abnormal α-1,4-G activity group(12 cases). Normal birth masculine seminal fluid matched ages was the control group (12cases). Neutral α-1,4-G activity in semen test kits was used to detect the neutral α-1,4-G activity . The positive rate and activity intensity of HYD in 2 groups were examined by our improved fixed—substrate film method. Results There was significant difference between normal birth group and infertile groups（P<0.01）. The relation between neutral α-1,4-G activity and the positive rate of HYD had a significant positive correlation (r=0.877,P<0.01); The relation between neutral α-1,4-G activity and the activity intensity of HYD showed a significant positive correlation (r=0.910,P<0.01). Conclusion The neutral α-1,4-G activity of human seminal plasma could affect the positive rate and activity intensity of HYD.