TWEAK促进大鼠心肌成纤维细胞增殖和胶原合成

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (2): 170-173.

TWEAK促进大鼠心肌成纤维细胞增殖和胶原合成

陈慧娜¹,任满意²,魏峰涛¹,卢淑霞¹,杨兆瑞¹,徐冬玲¹,王旭平³,隋树建⁴

1. 山东大学第二医院
2. 山东省胸科医院
3. 山东大学齐鲁医院
4. 山东大学第二医院心内科

收稿日期:2010-06-17 修回日期:2010-08-24 出版日期:2011-02-05 发布日期:2011-03-14
通讯作者: 隋树建 E-mail:suisj@163.com
基金资助:
省自然科学基金

TWEAK promotes the proliferation and collagen synthesis of rat cardiac fibroblasts

CHEN Hui-na ¹,REN Man-yi ²,WEI Feng-tao ²,LU Shu-xia ²,YANG Zhao-rui ²,XU Dong-ling ²,WANG Xu-ping ²,Shu-jian SUI,¹

1. the Second Hospital of Shandong University
2.

Received:2010-06-17 Revised:2010-08-24 Online:2011-02-05 Published:2011-03-14
Contact: Shu-jian SUI, E-mail:suisj@163.com

摘要/Abstract

摘要： 目的探讨肿瘤坏死因子样凋亡微弱诱导剂（TWEAK）对大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响。方法用胰酶消化法培养新生Wistar大鼠CFs，加入重组人TWEAK（rhTWEAK）干预48h，采用四甲基偶氮唑蓝(MTT)法测定细胞增殖，qRT-PCR检测Ⅰ型和Ⅲ型胶原mRNA，Western blot 检测Ⅰ型胶原蛋白表达。结果 rhTWEAK作用后CFs的细胞数目明显增加，I型和III型胶原mRNA表达上调，同时I型胶原蛋白表达显著增强。结论 TWEAK具有促进大鼠心肌成纤维细胞增殖和胶原合成的作用。

Abstract: Objective To investigate the effects of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) on the proliferation and collagen synthesis of cultured rat cardiac fibroblasts (CFs). Methods Cultured CFs from neonatal Wistar rats were isolated by trypsinization, treated with Recombinant human TWEAK (rhTWEAK), and examined after 48h. The CFs proliferation was examined by MTT, and the expressions of collagen Ⅰ and Ⅲ mRNA and protein were determined by qRT-PCR. The expression of collagen Ⅰ protein was determined by Western blot. Results rhTWEAK significantly increased the number of CFs and elevated the expression levels of collagenⅠand Ⅲ mRNA and collagenⅠprotein.Conclusion TWEAK can promote proliferation and collagen synthesis of rat CFs.

中图分类号:

陈慧娜任满意魏峰涛卢淑霞杨兆瑞徐冬玲王旭平隋树建. TWEAK促进大鼠心肌成纤维细胞增殖和胶原合成 [J]. 基础医学与临床, 2011, 31(2): 170-173.

CHEN Hui-na REN Man-yi WEI Feng-tao LU Shu-xia YANG Zhao-rui XU Dong-ling WANG Xu-ping Shu-jian SUI,. TWEAK promotes the proliferation and collagen synthesis of rat cardiac fibroblasts[J]. Basic & Clinical Medicine, 2011, 31(2): 170-173.