筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用

doi:10.16352/j.issn.1001-6325.2026.02.0208

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (2): 208-214.doi: 10.16352/j.issn.1001-6325.2026.02.0208

筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用

车彦霏, 覃东汕, 余姿漫, 王皓宇, 杨丹^*, 梁晓春^*

中国医学科学院北京协和医学院北京协和医院中医科转化医学中心,北京 100730

收稿日期:2025-11-25 修回日期:2025-12-15 出版日期:2026-02-05 发布日期:2026-01-21
通讯作者: ^* jsj000jsj@163.com; xiaochun_liang@yeah.net
基金资助:
国家自然科学基金(82004182)

Regulatory effects of Jinmaitong on high glucose-induced glycolytic reprogramming in RSC96 cells and polarization of RAW264.7 cells

CHE Yanfei, QIN Dongshan, YU Ziman, WANG Haoyu, YANG Dan^*, LIANG Xiaochun^*

Department of Traditional Chinese Medicine,Centre for Translational Medicine,Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100730,China

Received:2025-11-25 Revised:2025-12-15 Online:2026-02-05 Published:2026-01-21
Contact: ^* jsj000jsj@163.com; xiaochun_liang@yeah.net

摘要/Abstract

摘要： 目的探讨筋脉通对高糖诱导大鼠施万细胞系RSC96糖酵解,及其对巨噬细胞系RAW264.7极化的影响。方法将RSC96细胞构建为高糖代谢应激模型,分别给予不同浓度筋脉通干预,检测细胞增殖、乳酸含量、糖酵解关键酶(PKM2、LDHA)及组蛋白乳酸化修饰(H3K18la)蛋白表达,评估其对代谢与表观遗传的调控作用。进一步将高糖处理后RSC96细胞的条件培养上清作用于RAW264.7细胞,检测巨噬细胞极化相关表型指标,分析其免疫表型变化。结果高糖刺激可诱导RSC96细胞糖酵解通路活性增强和乳酸积聚,并上调组蛋白H3K18la乳酸化修饰水平,提示细胞处于代谢应激状态。筋脉通干预后可剂量依赖性降低糖酵解关键酶表达及乳酸水平,并抑制H3K18la修饰上调。巨噬细胞实验结果显示,高糖条件下施万细胞来源的条件培养上清可促进RAW264.7向M1型极化,筋脉通处理可逆转该趋势,诱导其向M2型转化,改善促炎微环境。结论高糖环境下,施万细胞发生糖酵解重编程并伴随组蛋白乳酸化修饰改变,可影响巨噬细胞极化,参与神经损伤进程。

关键词: 糖酵解重编程, 组蛋白乳酸化, 巨噬细胞极化, 筋脉通, 糖尿病周围神经病变

Abstract: Objective To investigate the regulatory effects of Jinmaitong on glycolysis and histone lactylation in high glucose-induced rat Schwann cell line RSC96, and its impact on macrophage polarization. Methods A high-glucose metabolic stress model was established using RSC96 cells, followed by treatment with different concentrations of Jinmaitong. Cell proliferation, lactate levels, and the expression of glycolytic enzymes (PKM2, LDHA) and histone lactylation marker H3K18la were assessed to evaluate metabolic and epigenetic changes. In addition, conditioned media from high-glucose-treated RSC96 cells were applied to RAW264.7 macrophages to examine changes in polarization-related phenotypic markers. Results High-glucose stimulation enhanced glycolytic activity and lactate accumulation in RSC96 cells, accompanied by upregulation of H3K18la modification, indicating a state of metabolic stress. Jinmaitong treatment led to a dose-dependent reduction in glycolytic enzyme expression and lactate levels, and significantly suppressed H3K18la expression. Conditioned media from high-glucose-treated Schwann cells promoted M1 polarization of RAW264.7 cells, whereas Jinmaitong intervention reversed this trend, promoting M2 polarization and alleviating the pro-inflammatory microenvironment. Conclusions High-glucose conditions induce glycolytic reprogramming and histone lactylation in Schwann cells, which may contribute to macrophage polarization and neural injury.

Key words: glycolytic reprogramming, histone lactylation, macrophage polarization, Jinmaitong (JMT), diabetic peripheral neuropathy

中图分类号:

R285.5
R969

车彦霏, 覃东汕, 余姿漫, 王皓宇, 杨丹, 梁晓春. 筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用[J]. 基础医学与临床, 2026, 46(2): 208-214.

CHE Yanfei, QIN Dongshan, YU Ziman, WANG Haoyu, YANG Dan, LIANG Xiaochun. Regulatory effects of Jinmaitong on high glucose-induced glycolytic reprogramming in RSC96 cells and polarization of RAW264.7 cells[J]. Basic & Clinical Medicine, 2026, 46(2): 208-214.

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筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用

Regulatory effects of Jinmaitong on high glucose-induced glycolytic reprogramming in RSC96 cells and polarization of RAW264.7 cells

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