关键词: 非小细胞肺癌, 长链非编码RNA(lnRNA), 线粒体RNA加工核糖核酸内切酶RNA组分(RMRP), 增殖, 侵袭

Abstract: Objective To investigate the effect of inhibiting the expression of RNA component of mitochondrial RNA processing endoribonuclease(RMRP) on the proliferation and invasion of human lung cancer cell line A549 in order to provide evidence to support the research on NSCLC mechanism. Methods A total of 122 cases of patients who underwent surgical treatment were selected in Jiaozuo People′s Hospital from March 2016 to March 2021. In the same period, 50 healthy people from physical examination center were selected as the control group. RT-qPCR was used to detect the expressions of RMRP in blood and tissues. Human lung cancer cell line A549 was cultured and divided into si-RMRP group, siRNA-NC group and blank group.RT-qPCR, CCK-8 and Transwell assays were used to detect the expression of RMRP, proliferation activity and cell counting of invasive cells. Results The relative expression level of RMRP in the blood of NSCLC patients was significantly higher than that of control group (P＜0.001). The relative expression level of RMRP in NSCLC tissues was significantly higher than the adjacent tissues (P＜0.001). The relative expression level of RMRP in blood and tissues of patients with poorly differentiated, lymph node metastasis and TNM stage Ⅲ compared with moderate to highly differentiated, no lymph node metastasis and TNM stage Ⅰ-Ⅱ were significantly increased (P＜0.05). The relative expression level of RMRP in the cells of the si-RMRP group was lower than that of blank and the siRNA-NC group (P＜0.001). Compared with the blank group and the siRNA-NC group, the absorbance (A) value of cells at 24, 48, 72 and 96 h in the si-RMRP group was decreased (P＜0.05). The number of invasion cells in the si-RMRP group was lower than that in the blank group and the siRNA-NC group (F=27.765, P＜0.001). Conclusions The relative expression levels of RMRP in blood and tissues of NSCLC patients are increased. Down-regulation of the expression of RMRP gene in A549 cells can inhibit cell proliferation and reduce cell invasion.

Key words: non-small cell lung cancer, long non-coding RNA(lnRNA), RNA component of mitochondrial RNA processing endoribonuclease(RMRP), proliferation, invasion