大蒜素抑制口腔癌细胞系CAL27的增殖

doi:10.16352/j.issn.1001-6325.2024.05.0673

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (5): 673-676.doi: 10.16352/j.issn.1001-6325.2024.05.0673

大蒜素抑制口腔癌细胞系CAL27的增殖

王婷婷^1,2#, 萧宁^3#, 林佃新⁴, 董海涛^3*

中国医学科学院北京协和医学院北京协和医院 1.临床生物样本中心;2.医学科学研究中心;3.口腔科,北京 100730;
4.莱钢集团莱芜矿业有限公司职工医院内科,山东济南 271100

收稿日期:2023-12-05 修回日期:2024-03-19 出版日期:2024-05-05 发布日期:2024-04-23
通讯作者: ^*jay41dong@126.com
^#对本文有相同贡献
基金资助:
中国医学科学院医学与健康科技创新工程项目(2022-I2M-C&T-B-022);中央高水平医院临床科研业务费(2022-PUMCH-C-070,2023-PUMCH-F-004)

Allicin inhibits the proliferation of oral cancer cells line CAL27

WANG Tingting^1,2#, XIAO Ning^3#, LIN Dianxin⁴, DONG Haitao^3*

1. Clinical Biobank; 2. Medical Research Center; 3. Department of Stomatology, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730;
4. Department of Internal Medicine, Laiwu Iron and Steel Group Laikuang Hospital, Jinan 271100, China

Received:2023-12-05 Revised:2024-03-19 Online:2024-05-05 Published:2024-04-23
Contact: ^*jay41dong@126.com

摘要/Abstract

摘要： 目的探讨大蒜素对口腔舌鳞状细胞癌(OTSCC)细胞系CAL27增殖的影响。方法采用人OTSCC细胞系CAL27,于培养液中加入不同浓度大蒜素(5、10、20、40、60、100、200、400 μmol/L)及对照DMSO处理后,进行CCK8法检测细胞增殖,RT-qPCR检测增殖相关标志物ki-67表达,以及转录本测序并进行通路富集分析。结果与对照组相比,较低浓度(20 μmol/L)的大蒜素即有抑制CAL27细胞增殖的作用(P＜0.01)。RT-qPCR结果显示,大蒜素能明显抑制CAL27细胞中ki-67表达,且在较低浓度(20 μmol/L)就有明显抑制作用(P＜0.01)。转录本测序结果显示,大蒜素能明显抑制肿瘤相关microRNA表达,尤其是miR-200a和miR-199a1的表达。结论较低浓度大蒜素即可明显抑制口腔癌细胞系CAL27的增殖,其可能通过抑制肿瘤相关microRNA来发挥作用。

关键词: 口腔癌, 大蒜素, 细胞增殖, 微RNA

Abstract: Objective To explore the effect of allicin on the proliferation of oral tongue squamous cell carcinoma(OTSCC) cells line(CAL27). Methods Human CAL27 cells were used in this study. The cells were incubated with different concentrations of allicin (5, 10, 20, 40, 60, 100, 200, 400 μmol/L) and control DMSO. CCK8 assay was performed to detect cell proliferation. RT-qPCR method was used to detect the expression of proliferation related marker ki-67; Transcriptome sequencing and pathway enrichment analysis were performed. Results Lower concentrations (20 μmol/L) of allicin inhibited CAL27 cells proliferation (P＜0.01).Allicin significantly inhibited the expression of ki-67 in CAL27 cells at lower concentrations(20 μmol/L, P＜0.01). Allicin inhibited tumor related microRNA expression, especially miR-200a and miR-199a1. Conclusions Lower concentration of allicin may significantly inhibit the proliferation of oral cancer cells by inhibition of tumor related microRNAs as a potential mechanism.

Key words: oral cancer, allicin, cell proliferation, microRNA

中图分类号:

王婷婷, 萧宁, 林佃新, 董海涛. 大蒜素抑制口腔癌细胞系CAL27的增殖[J]. 基础医学与临床, 2024, 44(5): 673-676.

WANG Tingting, XIAO Ning, LIN Dianxin, DONG Haitao. Allicin inhibits the proliferation of oral cancer cells line CAL27[J]. Basic & Clinical Medicine, 2024, 44(5): 673-676.

参考文献

[1] Campbell BR, Chen Z, Faden DL, et al. The mutational landscape of early- and typical-onset oral tongue squamous cell carcinoma[J]. Cancer, 2021,15:544-553.
[2] Almangush A, Heikkinen I, Makitie AA, et al. Progno-stic biomarkers for oral tongue squamous cell carcinoma: a systematic review and Meta-analysis[J]. Br J Cancer, 2017, 117:856-866.
[3] Ng JH, Iyer NG, Tan MH, et al. Changing epidemiology of oral squamous cell carcinoma of the tongue: a global study[J]. Head Neck, 2017, 39:297-304.
[4] Srinivasprasad V, Dineshshankar J, Sathiyajeeva J, et al. Liaison between micro-organisms and oral cancer[J]. J Pharm Bioallied Sci, 2015, 7: S354-S360.
[5] Cha JH, Choi YJ, Cha SH, et al. Allicin inhibits cell growth and induces apoptosis in U87MG human glioblastoma cells through an ERK-dependent pathway[J]. Oncol Rep, 2012, 28:41-48.
[6] Hirsch K, Danilenko M, Giat J, et al. Effect of purified allicin, the major ingredient of freshly crushed garlic, on cancer cell proliferation[J]. Nutr Cancer, 2000, 38:245-254.
[7] Song B, Shu Y, Cui T, Fu P. Allicin inhibits human renal clear cell carcinoma progression via suppressing HIF pathway[J]. Int J Clin Exp Med, 2015,8:20573-20580.
[8] Guo Y, Liu H, Chen Y, et al. The effect of allicin on cell proliferation and apoptosis compared to blank control and cis-platinum in oral tongue squamous cell carcinoma[J]. OncoTargets Therapy, 2020,13:13183-13189.
[9] Lee YS, Dutta A. MicroRNAs in cancer[J]. Review Annu Rev Pathol, 2009, 4:199-227.
[10] Wang L, Wang Q, Li HL, et al. Expression of miR200a, miR93, metastasis-related gene RECK and MMP2/MMP9 in human cervical carcinoma--relationship with prognosis[J]. Asian Pac J Cancer Prev, 2013,14:2113-2118.
[11] Chen BF, Suen YK, Gu S, et al. A miR-199a/miR-214 self-regulatory network via PSMD10, TP53 and DNMT1 in testicular germ cell tumor[J]. Sci Rep, 2014, 18:6413. doi:10.1038/srep06413.

[1]	姚安军, 陈凌子, 金惠仙. 二十二碳六烯酸抑制人结肠癌细胞系HT-29增殖[J]. 基础医学与临床, 2024, 44(8): 1107-1112.
[2]	曹清文, 祁琳, 禹博, 田晨晨, 袁海宁, 王越. 红景天苷促进人血管内皮细胞系EA.hy926增殖与迁移[J]. 基础医学与临床, 2024, 44(7): 925-930.
[3]	张鹏举, 李杰, 张梦迪, 孙少科, 许寅喆. KAT8通过增强METTL3表达促进结直肠癌细胞系增殖[J]. 基础医学与临床, 2024, 44(7): 931-939.
[4]	高寅洁, 童安莉. 醛固酮产生腺瘤中细胞死亡和增殖机制[J]. 基础医学与临床, 2024, 44(6): 758-762.
[5]	梁香存, 魏小雨, 梁健, 王庆, 耿广. 安罗替尼通过调控miR-16-5p/PD-1轴抑制人非小细胞肺癌细胞系增殖并促进凋亡[J]. 基础医学与临床, 2024, 44(4): 503-512.
[6]	陆凯, 陆建菊, 郭文利, 黄建棋, 李志华. lncRNA VIM-AS5在人乳腺癌细胞系中的表达及对乳腺癌细胞增殖和迁移的影响[J]. 基础医学与临床, 2024, 44(4): 447-453.
[7]	李娜, 李涛, 杨冬梨, 姚媛. 和厚朴酚抑制人脂肪间充质干细胞增殖[J]. 基础医学与临床, 2024, 44(4): 483-488.
[8]	贾安娜, 战世佳, 张璇, 郭金鑫, 于永波, 郭永丽, 常艳. C12ORF66对MYCN扩增的高危神经母细胞瘤细胞的活性调控[J]. 基础医学与临床, 2024, 44(3): 288-294.
[9]	廖镇城, 杨思懿, 吴平安. 提高m⁶A去甲基化酶FTO表达抑制鼻咽癌细胞增殖[J]. 基础医学与临床, 2024, 44(1): 57-62.
[10]	余洁, 马明磊, 张化冰, 平凡, 李伟, 许岭翎, 李玉秀. miR-139-5p增强二甲双胍抑制正常糖培养下人胰腺癌细胞系PANC-1增殖[J]. 基础医学与临床, 2024, 44(1): 8-15.
[11]	潘闪, 林铿强, 陈树兴. 培美曲塞抑制人肺腺癌细胞系A549增殖[J]. 基础医学与临床, 2023, 43(9): 1359-1363.
[12]	梁辉勇, 韦秋慧, 丁亚, 彭小青, 姚静, 苏齐鉴. 虫草素抑制TNF-α诱导的人角质形成细胞系HaCaT增殖[J]. 基础医学与临床, 2023, 43(9): 1390-1393.
[13]	张培波, 李义. 沙棘总黄酮抑制膀胱癌细胞系T24增殖[J]. 基础医学与临床, 2023, 43(7): 1122-1126.
[14]	侯孟杰, 赵娜, 黄富强, 王亚南, 刘芳铭. 载脂蛋白E通过降低高尔基体膜蛋白73表达抑制小鼠肝癌细胞系增殖[J]. 基础医学与临床, 2023, 43(5): 762-770.
[15]	王芳芳, 曹慧媛, 汪婧, 王宁, 黄国良. 上调miR-200c-5p的表达抑制人结直肠癌细胞系SW480增殖[J]. 基础医学与临床, 2023, 43(2): 241-245.

大蒜素抑制口腔癌细胞系CAL27的增殖

Allicin inhibits the proliferation of oral cancer cells line CAL27

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价