长链非编码RNA亚细胞定位和功能的研究进展

doi:10.16352/j.issn.1001-6325.2023.10.1580

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (10): 1580-1584.doi: 10.16352/j.issn.1001-6325.2023.10.1580

长链非编码RNA亚细胞定位和功能的研究进展

苏越^1,2, 梁琳慧^1,2*, 何祥火^1,2*

1.复旦大学生物医学研究院,上海 200032;
2.复旦大学附属肿瘤医院肿瘤研究所,上海 200032

收稿日期:2023-02-27 修回日期:2023-04-11 出版日期:2023-10-05 发布日期:2023-10-05
通讯作者: *lianglinhui@fudan.edu.cn; xhhe@fudan.edu.cn
基金资助:
国家自然科学基金(81972253)

Research progress on subcellular localization and function of long non-coding RNA

SU Yue^{1, 2}, LIANG Linhui^{1, 2*}, HE Xianghuo^{1, 2*}

1. Institutes of Biomedical Sciences,Fudan University,Shanghai 200032;
2. Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China

Received:2023-02-27 Revised:2023-04-11 Online:2023-10-05 Published:2023-10-05
Contact: *lianglinhui@fudan.edu.cn; xhhe@fudan.edu.cn

摘要/Abstract

摘要： 长链非编码RNA(lncRNA)的亚细胞定位和其功能息息相关,定位于细胞核时,lncRNA可以维持染色质结构,调控基因转录,参与mRNA的可变剪接;定位于细胞质时参与信号传导、转录后调控、翻译和翻译后修饰;定位于细胞器时协助完成细胞器的功能。lncRNA的亚细胞定位机制与其自身序列、结合蛋白等密切相关。此外通过构建核滞溜载体Snovector、添加胞质定位元件等强制改变lncRNA的亚细胞定位,以及利用APEX2等技术研究lncRNA亚细胞定位和其功能之间的关系,有利于lncRNA疗法的开发。

关键词: 长链非编码RNA, 亚细胞定位, 长链非编码RNA功能

Abstract: The subcellular localization of long non-coding RNA(lncRNA) is closely related to its function. When localized in the nucleus, lncRNA can maintain chromatin structure, regulate gene transcription and participate in variable splicing of mRNA. When localized in the cytoplasm, lncRNA can mediate signal transduction, post-transcriptional regulation, translation and post-translational modification. When localized in the organelles, lncRNA can assist in the function of them. The subcellular localization of lncRNA is closely related to its own sequences and binding proteins. In addition, the development of lncRNA therapy will be facilitated by changing lncRNA subcellular localization in use of constructing Snovector, adding cytosolic localization elements, or techniques like APEX2, which indicates the relationship between the subcellular localization of lncRNA and its function.

Key words: long non-coding RNA, subcellular localization, function of long non-coding RNA

中图分类号:

苏越, 梁琳慧, 何祥火. 长链非编码RNA亚细胞定位和功能的研究进展[J]. 基础医学与临床, 2023, 43(10): 1580-1584.

SU Yue, LIANG Linhui, HE Xianghuo. Research progress on subcellular localization and function of long non-coding RNA[J]. Basic & Clinical Medicine, 2023, 43(10): 1580-1584.

参考文献 24

[1]	Gao Y, Shang S, Guo S, et al. Lnc2Cancer 3.0: an updated resource for experimentally supported lncRNA/circRNA cancer associations and web tools based on RNA-seq and scRNA-seq data[J]. Nucleic Acids Res, 2021, 49:D1251-D1258.
[2]	Fazal FM, Han S, Parker KR, et al. Atlas of subcellular RNA localization revealed by APEX-Seq[J]. Cell, 2019, 178:473-490.e426.
[3]	Sang L, Ju HQ, Yang Z, et al. Mitochondrial long non-coding RNA GAS5 tunes TCA metabolism in response to nutrient stress[J]. Nat Metab, 2021, 3:90-106.
[4]	Yin QF, Hu SB, Xu YF, et al. SnoVectors for nuclear expression of RNA[J]. Nucleic Acids Res, 2015, 43:e5.doi: 10.1093/nar/gku1050.
[5]	Truong DJ, Armbrust N, Geilenkeuser J, et al. Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs[J]. Nat Cell Biol, 2022, 24:1666-1676.
[6]	Khan M, Hou S, Azam S, et al. Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway[J]. Nucleic Acids Res, 2021, 49:6420-6436.
[7]	Guo CJ, Ma XK, Xing YH, et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell, 2020, 181:621-636.e622.
[8]	Feretzaki M, Pospisilova M, Valador Fernandes R, et al. RAD51-dependent recruitment of TERRA lncRNA to telomeres through R-loops[J]. Nature, 2020, 587:303-308.
[9]	Kaminski N, Wondisford AR, Kwon Y, et al. RAD51AP1 regulates ALT-HDR through chromatin-directed homeo-stasis of TERRA[J]. Mol Cell, 2022, 82:4001-4017.e4007.
[10]	Simion V, Zhou H, Haemmig S, et al. A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus[J]. Nat Commun, 2020, 11:6135. doi: 10.1038/s41467-020-19664-2.
[11]	Larrasa-Alonso J, Villalba-Orero M, Martí-Gómez C, et al. The SRSF4-GAS5-glucocorticoid receptor axis regulates ventricular hypertrophy[J]. Circ Res, 2021, 129:669-683.
[12]	Huan L, Guo T, Wu Y, et al. Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response[J]. Mol Cancer, 2020, 19:11. doi: 10.1186/s12943-019-1122-z.
[13]	Liang L, Huan L, Wang J, et al. LncRNA RP11-295G20.2 regulates hepatocellular carcinoma cell growth and autophagy by targeting PTEN to lysosomal degradation[J]. Cell Discov, 2021, 7:118. doi: 10.1038/s41421-021-00339-1.
[14]	Jia Y, Tian C, Wang H, et al. Long non-coding RNA NORAD/miR-224-3p/MTDH axis contributes to CDDP resistance of esophageal squamous cell carcinoma by promoting nuclear accumulation of β-catenin[J]. Mol Cancer, 2021, 20:162. doi: 10.1186/s12943-021-01455-y.
[15]	Sato M, Kadomatsu T, Miyata K, et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis[J]. Nat Commun, 2021, 12:2529. doi: 10.1038/s41467-021-22735-7.
[16]	Song TJ, Ke J, Chen F, et al. Effect of SNHG11/miR-7-5p/PLCB1 axis on acute pancreatitis through inhibiting p38MAPK pathway[J]. Cells, 2022, 12:65. doi: 10.3390/cells12010065.
[17]	Xu L, Huan L, Guo T, et al. LncRNA SNHG11 facili-tates tumor metastasis by interacting with and stabilizing HIF-1α[J]. Oncogene, 2020, 39:7005-7018.
[18]	Hansji H, Leung EY, Baguley BC, et al. ZFAS1: a long noncoding RNA associated with ribosomes in breast cancer cells[J]. Biol Direct, 2016, 11:62. doi: 10.1186/s13062-016-0165-y.
[19]	Nguyen TM, Kabotyanski EB, Reineke LC, et al. The SINEB1 element in the long non-coding RNA Malat1 is necessary for TDP-43 proteostasis[J]. Nucleic Acids Res, 2020, 48:2621-2642.
[20]	Zuckerman B, Ron M, Mikl M, et al. Gene architecture and sequence composition underpin selective dependency of nuclear export of long RNAs on NXF1 and the TREX complex[J]. Mol Cell, 2020, 79:251-267.e256.
[21]	Noh JH, Kim KM, Abdelmohsen K, et al. HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP[J]. Genes Dev, 2016, 30:1224-1239.
[22]	Park MK, Zhang L, Min KW, et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis[J]. Cell Metab, 2021, 33:2380-2397.e2389.
[23]	Tran KV, Brown EL, DeSouza T, et al. Human thermogenic adipocyte regulation by the long noncoding RNA LINC00473[J]. Nat Metab, 2020, 2:397-412.
[24]	Padmakumar S, Jones G, Pawar G, et al. Minimally invasive nasal depot (MIND) technique for direct BDNF AntagoNAT delivery to the brain[J]. J Control Release, 2021, 331:176-186.

长链非编码RNA亚细胞定位和功能的研究进展

Research progress on subcellular localization and function of long non-coding RNA

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