肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

doi:10.16352/j.issn.1001-6325.2022.12.1861

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (12): 1861-1866.doi: 10.16352/j.issn.1001-6325.2022.12.1861

肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

杨晔^1*, 党荣广¹, 张静¹, 董超¹, 宋雪晶²

石家庄市人民医院 1.肿瘤科一病区; 2.肿瘤科二病区,河北石家庄 050000

收稿日期:2021-12-13 修回日期:2022-05-09 出版日期:2022-12-05 发布日期:2022-11-23
通讯作者: ^* yang1986ye@163.com
基金资助:
河北省卫生健康委员会资助项目(20191462)

Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients

YANG Ye^1*, DANG Rong-guang¹, ZHANG Jing¹, DONG Chao¹, SONG Xue-jing²

1. Oncology Department 1st Ward; 2. Oncology Department 2nd Ward, Shijiazhuang People's Hospital, Shijiazhuang 050000, China

Received:2021-12-13 Revised:2022-05-09 Online:2022-12-05 Published:2022-11-23
Contact: ^* yang1986ye@163.com

摘要/Abstract

摘要： 目的探究肺癌组织中长链非编码RNA锌指结构反义转录本1(lncRNA ZFAS1)、hsa-miR-372-3p表达水平及与患者恶性特征及预后的关系。方法选取2015年3月至2016年10月在石家庄市人民医院诊治的82例肺癌患者。RT-qPCR检测肿瘤组织和癌旁组织ZFAS1和miR-372-3p的表达,分析ZFAS1、miR-372-3p水平与肺癌患者恶性特征的关系;Pearson分析肿瘤组织中ZFAS1和miR-372-3p的关系; 用Kaplan-Meier分析ZFAS1、miR-372-3p水平与肺癌患者预后生存期的关系;COX回归分析对肺癌患者预后产生影响的因素。结果与癌旁组织相比,肿瘤组织中ZFAS1、miR-372-3p表达水平均显著升高(P＜0.05)。肿瘤组织ZFAS1、miR-372-3p表达水平与TNM分期、浸润胸膜、是否发生淋巴结转移、肿瘤分化程度有关(P＜0.05)。肿瘤组织中ZFAS1和miR-372-3p表达水平呈显著正相关(r=0.477,P＜0.001)。ZFAS1和miR-372-3p高表达组患者5年内生存率均明显低于低表达组(P＜0.05)。TNM分期、淋巴结转移、ZFAS1、miR-372-3p是肺癌患者发生不良预后的独立危险因素(P＜0.05)。结论肿瘤组织中ZFAS1、miR-372-3p呈异常高表达,其高水平与肺癌患者恶性特征及预后不良有关,是肺癌患者预后不良的独立危险因素。

关键词: 肺癌, 长链非编码RNA, 锌指结构反义转录本1, hsa-miR-372-3p

Abstract: Objective To study the correlation of the expression of long non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1) and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients. Methods Eighty-two lung cancer patients hospitalized in Shijiazhuang People's Hospital from March 2015 to October 2016 were selected. The expression of ZFAS1 and miR-372-3p in lung cancer tissues and in adjacent tissues, the potential correlation of the level of ZFAS1 and miR-372-3p with malignant characteristics of patients were analyzed; Pearson method was used to analyze the relationship between ZFAS1 and miR-372-3p in lung cancer tissues; Kaplan-Meier was used to analyze the correlation of the expression of ZFAS1 and miR-372-3p with the prognosis of patients; COX regression was used to analyze the risk factors for poor prognosis of lung cancer patients. Results Compared with the situation of adjacent tissues, the expression of ZFAS1 and miR-372-3p in tumor tissue was significantly higher (P＜0.05). The expression of ZFAS1 and miR-372-3p in tumor tissue was related to TNM staging, lung membrane infiltration, whether lymph node metastasis occurred, and the degree of tumor differentiation (P＜0.05). The expression of ZFAS1 and miR-372-3p content in lung cancer tissues was significantly and positively correlated (r=0.477, P＜0.001). The 5-year survival rate of patients in the ZFAS1 and miR-372-3p high expression group was significantly lower than the low expression group (P＜0.05). TNM staging, lymph node metastasis, ZFAS1, and miR-372-3p were independent risk factors for poor prognosis in patients with lung cancer(P＜0.05). Conclusions ZFAS1 and miR-372-3p are highly expressed in lung cancer tissues and the expression level is related to the malignant characteristics and prognosis of lung cancer patients, so it may be regarded as an independent risk factor to predict poor prognosis of lung cancer patients.

Key words: lung cancer, long non-coding RNA, zinc finger antisense 1, hsa-miR-372-3p

中图分类号:

R734.2

杨晔, 党荣广, 张静, 董超, 宋雪晶. 肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系[J]. 基础医学与临床, 2022, 42(12): 1861-1866.

YANG Ye, DANG Rong-guang, ZHANG Jing, DONG Chao, SONG Xue-jing. Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients[J]. Basic & Clinical Medicine, 2022, 42(12): 1861-1866.

参考文献 14

[1]	潘瑞丽,马千惠,徐燕,等.希望水平在晚期肺癌患者抑郁状态与心理韧性中的中介效应[J].基础医学与临床,2022,42:291-295.
[2]	张千, 朱亚宁, 周武碧. 长链非编码RNA ZFAS1在食管癌中的表达及作用机制研究[J].转化医学杂志, 2020, 9:326-330+350.
[3]	彭胜利,肖婷,彭开利,等.LncRNA ZFAS1通过靶基因miR-432-5p调控肺癌细胞增殖、侵袭和迁移的分子机制[J].中国老年学杂志,2021,41:5688-5693.
[4]	张伟, 郭永连, 李国灏, 等. miR-372-3p在膀胱癌组织中的表达及其对膀胱癌细胞增殖、迁移与侵袭的影响[J]. 中国肿瘤生物治疗杂志, 2018, 25:45-50.
[5]	Fan X, Huang X, Li Z, et al. MicroRNA-372-3p promotes the epithelial-mesenchymal transition in breast carcinoma by activating the Wnt pathway[J]. J BUON. 2018, 23:1309-1315.
[6]	Fan J, Zhang J, Huang S, et al. LncRNA OSER1-AS1 acts as a ceRNA to promote tumorigenesis in hepato-cellular carcinoma by regulating miR-372-3p/Rab23 axis[J]. Biochem Biophys Res Commun, 2020, 521:196-203.
[7]	杨汶川,韩东,杨帆.LINC00607通过抑制mi R-372影响A549/DDP细胞的增殖和凋亡[J].基础医学与临床,2021,41:1001-1006.
[8]	Li Z, Qin X, Bian W, et al. Exosomal lncRNA ZFAS1 regulates esophageal squamous cell carcinoma cell proliferation, invasion, migration and apoptosis via microRNA-124/STAT3 axis[J]. J Exp Clin Cancer Res, 2019, 38:477-489.
[9]	Dong D, Mu Z, Zhao C, et al. ZFAS1: a novel tumor-related long non-coding RNA[J]. Cancer Cell Int, 2018, 18:125-132.
[10]	Zeng Z, Zhao G, Rao C, et al. Knockdown of lncRNA ZFAS1-suppressed non-small cell lung cancer progression via targeting the miR-150-5p/HMGA2 signaling[J]. J Cell Biochem, 2019, 6:1-11.
[11]	Fan G, Jiao J, Shen F, et al. Upregulation of lncRNA ZFAS1 promotes lung adenocarcinoma progression by sponging miR-1271-5p and upregulating FRS2[J]. Thorac Cancer, 2020, 11:2178-2187.
[12]	Gao Y, Ma H, Gao Y, et al. circRNA Circ_0001721 promotes the progression of osteosarcoma through miR-372-3p/MAPK7 axis[J]. Cancer Manag Res, 2020, 12:8287-8302.
[13]	Wang Q, Liu S, Zhao X, et al. MiR-372-3p promotes cell growth and metastasis by targeting FGF9 in lung squamous cell carcinoma[J]. Cancer Med, 2017, 6:1323-1330.
[14]	Sun H, Gao D. Propofol suppresses growth, migration and invasion of A549 cells by down-regulation of miR-372[J]. BMC Cancer, 2018, 18:1252-1262.

[1]	郑红, 陈晓霞, 韩李周, 陈苗, 皇甫娟. 降低lncRNA-RMRP表达抑制人肺癌细胞系A549的增殖和侵袭[J]. 基础医学与临床, 2024, 44(7): 974-978.
[2]	操辰新, 唐辉, 耿瑞璇, 郭伏平, 白春梅, 王颖轶, 李太生. 循环CD4⁺CD45RA⁺CD62L⁺ T细胞与接受EGFR-TKI治疗的转移性非小细胞肺癌预后相关[J]. 基础医学与临床, 2024, 44(5): 658-664.
[3]	梁香存, 魏小雨, 梁健, 王庆, 耿广. 安罗替尼通过调控miR-16-5p/PD-1轴抑制人非小细胞肺癌细胞系增殖并促进凋亡[J]. 基础医学与临床, 2024, 44(4): 503-512.
[4]	张婧, 杨自更, 蔡文琴, 曹维维, 韦红梅, 薛茜茜, 吴宾. 抑制抗增殖蛋白2增强非小细胞肺癌细胞系A549对厄洛替尼敏感性[J]. 基础医学与临床, 2024, 44(3): 325-332.
[5]	李娟, 李晓峰, 徐生志, 唐凯. 华蟾素胶囊联合多西紫杉醇+顺铂化疗用于非小细胞肺癌患者的疗效[J]. 基础医学与临床, 2024, 44(2): 247-251.
[6]	丁飞, 王洒, 黄常新. 非小细胞肺癌患者血清CA211水平和NK细胞数与预后相关[J]. 基础医学与临床, 2024, 44(2): 180-184.
[7]	张伟, 张振, 杨连任, 梁伟. 肺癌7项抗体水平与NSCLC患者化疗效果及预后的关系[J]. 基础医学与临床, 2024, 44(10): 1383-1387.
[8]	王春燕, 王萍, 宋龙飞, 刘永全, 满君. LncRNA FEZF1-AS1通过调控EZH2对肺间质细胞增殖、迁移及侵袭的作用[J]. 基础医学与临床, 2024, 44(1): 43-50.
[9]	颜晨红, 金儿. 外泌体PD-L1在非小细胞肺癌诊断和治疗上的研究进展[J]. 基础医学与临床, 2023, 43(9): 1457-1461.
[10]	张小婉, 康霞, 姚小英, 谢芳, 李莹. 沉默SF3B1促进人肺癌细胞系A549凋亡并抑制其增殖及侵袭[J]. 基础医学与临床, 2023, 43(8): 1265-1270.
[11]	李珍, 段昭君, 罗云萍. 神经节苷脂GD2可作为肺癌干细胞一个潜在的候选标志物[J]. 基础医学与临床, 2023, 43(5): 777-784.
[12]	薛剑超, 王亚东, 李博文, 刘新宇, 梁乃新, 李单青. DNA甲基化在肺癌发生及预后中作用的研究进展[J]. 基础医学与临床, 2023, 43(3): 514-518.
[13]	张婧, 杨自更, 韦红梅, 宁海虹, 薛茜茜, 金玲, 吴宾. 敲减抗增殖蛋白2促进非小细胞肺癌细胞系A549凋亡[J]. 基础医学与临床, 2023, 43(10): 1522-1529.
[14]	苏越, 梁琳慧, 何祥火. 长链非编码RNA亚细胞定位和功能的研究进展[J]. 基础医学与临床, 2023, 43(10): 1580-1584.
[15]	邱立彬, 张新才, 赵倩. LncRNA DANCR靶向miR-3646减轻缺氧诱导的大鼠心肌细胞系H9c2的损伤[J]. 基础医学与临床, 2023, 43(1): 144-151.

肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 14

相关文章 15

编辑推荐

Metrics

本文评价