蛇菰多糖减轻急性脊髓挫伤模型大鼠的神经元损伤

doi:10.16352/j.issn.1001-6325.2022.10.1509

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1509-1513.doi: 10.16352/j.issn.1001-6325.2022.10.1509

蛇菰多糖减轻急性脊髓挫伤模型大鼠的神经元损伤

赵方毓^1,2, 覃晓莉², 何一多¹, 王凤杰^1,2, 唐鲜娥^1,2, 彭新鑫³, 陈显兵^1,2*

1.湖北民族大学附属民大医院病理科,湖北恩施 445000;
2.湖北民族大学医学部, 湖北恩施 445000;
3.湖北恩施学院医学院, 湖北恩施 445000

收稿日期:2021-07-28 修回日期:2022-01-25 出版日期:2022-10-05 发布日期:2022-09-23
通讯作者: * chenxianbing7612@163.com
基金资助:
湖北省教育厅科学技术研究计划青年人才项目(Q20181905);湖北民族大学博士启动基金(MY2017B004,MD2020B014)

Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury

ZHAO Fang-yu^1,2, QIN Xiao-li², HE Yi-duo¹, WANG Feng-jie^1,2, TANG Xian-e^1,2, PENG Xin-xin³, CHEN Xian-bing^1,2*

1. Department of Pathology,Minda Hospital of Hubei Minzu University, Enshi 445000;
2. Department of Medicine, Hubei Minzu University, Enshi 445000;
3. College of Medicine, Hubei Enshi College,Enshi 445000,China

Received:2021-07-28 Revised:2022-01-25 Online:2022-10-05 Published:2022-09-23
Contact: * chenxianbing7612@163.com

摘要/Abstract

摘要： 目的探讨蛇菰多糖(BPS)对神经自噬的调节及对急性脊髓挫伤(SCI)的治疗作用。方法将大鼠分为假手术组(sham,n=10)、模型组(SCI,n=15,用改良Allen's脊髓撞击法)和BPS干预组(BPS,n=45,灌胃给予低、中、高3个剂量14 d);用BBB评分评价运动功能;采用HE及Nissl染色法观察形态;蛋白质免疫印迹法检测自噬相关蛋白的表达变化。结果与sham组相比,SCI组大鼠的BBB评分-时间变化曲线明显降低,损伤程度达重度(P＜0.05);SCI的脊髓背侧白质和中央灰质均损伤严重,空洞明显;神经元数量明显减少,胞体结构不完整;SCI组第1、7、14天的LC3 Ⅱ/Ⅰ和p62相对表达水平上调,其中第7天表达最高(P＜0.05);而Beclin 1表达整体下调,于第7天表达最低(P＜0.05);与SCI组比较,BPS组大鼠BBB评分曲线呈剂量依赖性明显增高;BPS组背侧白质和中央灰质空洞缩小,神经元数量多,形态完整;BPS组Beclin 1、LC3Ⅱ表达明显回升,而p62蛋白较SCI组下调(P＜0.05)。结论蛇菰多糖对大鼠具有明确的神经保护作用,其作用机制可能与保护损伤的神经细胞有关。

关键词: 蛇菰多糖, 脊髓损伤, 自噬

Abstract: Objective To investigate the effect of balanophora polysaccharide (BPS) on neuro-autophagy and its therapeutic effect on spinal cord injury. Methods The rats were randomly divided into sham operation group(sham, n=10),model group(SCI, n=15,which were established according to the Allen's weight-striking method), and the BPS treatment group(BPS, n=45),which were given intra-gastrically with low, medium and high dose of BPS solution for 14 days). The BBB scores were measured to evaluate motor function. Hematoxylin-Eosin and Nissl staining was used to observe the morphological changes. The expression of autophagy markers of Beclin 1, LC3-Ⅱ and p62 was detected by Western blot. Results Compared with the sham operation group,the curve of BBB score-time in SCI group was significantly lower and the injury was severer(P＜0.05). In SCI group, the dorsal white matter and central gray matter of spinal cord were seriously injured with obvious cavity. Further observation showed that the number of neurons in SCI group was significantly decreased; the structure of cell body was incomplete. The result of Western bolt showed that the relative change of LC3 Ⅱ /Ⅰ and p62 was up-regulated on day 1,7 and 14, the highest expression of both protein was found on day 7(P＜0.05). At the same time,Beclin 1 was decreased after injury with lowest expression on day 7(P＜0.05). The BBB scores of BPS group were increased with a dose-dependent manner as compared with SCI group. The cavity of dorsal white matter and central gray matter shrinked. The number of neurons was increased with intact morphology. The expression of Beclin 1 and LC3 Ⅱ/Ⅰ was increased significantly in BPS group, while p62 decreased as compared to SCI group(P＜0.05). Conclusions BPS has a well-demonstrated neuron-protective effect and the underlying mechanism might be related to the protection of injured nerve cells.

Key words: Balanophora polysaccharide, spinal cord injury, autophagy

中图分类号:

R285.5

赵方毓, 覃晓莉, 何一多, 王凤杰, 唐鲜娥, 彭新鑫, 陈显兵. 蛇菰多糖减轻急性脊髓挫伤模型大鼠的神经元损伤[J]. 基础医学与临床, 2022, 42(10): 1509-1513.

ZHAO Fang-yu, QIN Xiao-li, HE Yi-duo, WANG Feng-jie, TANG Xian-e, PENG Xin-xin, CHEN Xian-bing. Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury[J]. Basic & Clinical Medicine, 2022, 42(10): 1509-1513.

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蛇菰多糖减轻急性脊髓挫伤模型大鼠的神经元损伤

Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury

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