CircRNA_23113抑制肺腺癌细胞的增殖和迁移

doi:10.16352/j.issn.1001-6325.2022.01.012

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (1): 82-88.doi: 10.16352/j.issn.1001-6325.2022.01.012

CircRNA_23113抑制肺腺癌细胞的增殖和迁移

王灵霞^1,2, 严玉兰^1*, 袁海涛³, 蔡烨伟³, 刘德慧¹, 谷文露¹, 曹碧月¹

江苏大学附属人民医院 1.呼吸内科;
3.普外科, 江苏镇江 212002;
2.上海市松江区中心医院呼吸内科,上海 201600

收稿日期:2020-12-04 修回日期:2021-05-10 出版日期:2022-01-05 发布日期:2022-01-05
通讯作者: ^* ylyan2005@163.com
基金资助:
江苏省自然科学基金(BK20151333);镇江市重点研发计划(SH2018059)

CircRNA_23113 inhibits the proliferation and migration of lung adenocarcinoma cells

WANG Ling-xia^1,2, YAN Yu-lan^1*, YUAN Hai-tao³, CAI Ye-wei³, LIU De-hui¹, GU Wen-lu¹, CAO Bi-yue¹

1. Department of Respiratory Medicine;
3. Department of General Surgery, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002;
2. Department of Respiratory Medicine, Shanghai Songjiang District Central Hospital, Shanghai 201600, China

Received:2020-12-04 Revised:2021-05-10 Online:2022-01-05 Published:2022-01-05
Contact: ^* ylyan2005@163.com

摘要/Abstract

摘要： 目的探究circRNA_23113在肺腺癌中的表达及对肺腺癌细胞增殖和迁移的影响。方法通过高通量测序技术测序5例临床肺腺癌、癌旁组织标本,筛选出差异性表达的circRNA_23113。实时荧光定量PCR检测肺腺癌组织、血清和细胞中circRNA_23113的表达;构建circRNA_23113的过表达质粒,用CCK-8法、克隆形成实验、细胞划痕和Transwell小室法分析其对细胞增殖和迁移的影响;用Western blot 检测β-catenin、cyclin D1以及c-myc蛋白水平的表达。结果 CircRNA_23113在肺腺癌患者组织、血清和细胞中显著低表达(P＜0.05);过表达circRNA_23113后抑制A549和H1299细胞增殖和迁移(P＜0.05);CircRNA_23113抑制β-catenin、cyclin D1以及c-myc表达(P＜0.05)。结论 CircRNA_23113在肺腺癌中低表达,且过表达能抑制肺腺癌细胞的增殖和迁移。

关键词: circRNA_23113, 肺腺癌, 增殖, 迁移

Abstract: Objective To explore the expression of circRNA_23113 in lung adenocarcinoma and its influence on the proliferation/migration of the adenocarcinoma cells. Methods Screening 5 cases of lung adenocarcinoma and adjacent tissues by high-throughput sequencing from which screened out the differentially expressed circular RNA circRNA_23113. Real-time fluorescence quantitative PCR was used to detect the expression of circRNA_23113 in the adenocarcinoma tissues, serum and cells; the over-expression plasmid of circRNA_23113 was constructed, and its effect on cell proliferation and migration was measured by CCK-8 method, clone formation experiment, cell scratching and Transwell chamber method; Western blot was used to detect the expression of β-catenin, cyclin D1 and c-myc protein. Results CircRNA_23113 was significantly less expressed in tissues, serum and cells of patients with lung cancer (P＜0.05); Over-expression of circRNA_23113 inhibited the proliferation and migration of A549 and H1299 cells(P＜0.05); CircRNA_23113 inhibited the expression of β-catenin, cyclin D1 and c-myc(P＜0.05). Conclusions CircRNA_23113 is poorly expressed in lung adenocarcinoma, and over-expression may inhibit the proliferation and migration of lung adenocarcinoma cells.

Key words: circRNA_23113, lung adenocarcinoma, proliferation, migration

中图分类号:

R734.2

王灵霞, 严玉兰, 袁海涛, 蔡烨伟, 刘德慧, 谷文露, 曹碧月. CircRNA_23113抑制肺腺癌细胞的增殖和迁移[J]. 基础医学与临床, 2022, 42(1): 82-88.

WANG Ling-xia, YAN Yu-lan, YUAN Hai-tao, CAI Ye-wei, LIU De-hui, GU Wen-lu, CAO Bi-yue. CircRNA_23113 inhibits the proliferation and migration of lung adenocarcinoma cells[J]. Basic & Clinical Medicine, 2022, 42(1): 82-88.

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CircRNA_23113抑制肺腺癌细胞的增殖和迁移

CircRNA_23113 inhibits the proliferation and migration of lung adenocarcinoma cells

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