关键词: 布托啡诺, 乳腺癌, PBX3, 增殖, 迁移, 侵袭

Abstract: Objective To investigate the effects of butorphanol on proliferation, migration and invasion of breast cancer cell line MCF7 and its related molecular mechanisms. Methods MTT assay was used to detect the inhibitory effect of different concentrations of butorphanol on human breast cancer MCF7 cell proliferation. Transwell migration and invasion assay were used to detect the effects of different concentrations of butorphanol on migration and invasion of human breast cancer MCF7 cells. RT-qCR and Western blot were used to detect PBX3 mRNA and protein expression in breast cancer cell lines, normal breast epithelial cells and butorphanol-treated MCF7 cells, respectively. MCF7 cells were transfected with si-PBX3 or si-con, and the changes of cell proliferation, migration and invasion were observed. PBX3 overexpression was used to verify the effect of butorphanol on proliferation, migration and invasion of breast cancer. The expressions of cyclin D1 and MMP-2 protein were detected by Western blot. Results The expression of PBX3 was up-regulated in breast cancer cell lines as compared to normal breast epithelial cells, and silencing PBX3 expression significantly inhibited the proliferation, migration and invasion of MCF7 cells,and inhibited the expression of cyclin D1 and MMP-2. Different concentrations of butorphanol significantly inhibited the proliferation, migration and invasion of MCF7 cells in a concentration-dependent manner, and inhibited the expression of PBX3, cyclin D1 and MMP-2. Overexpression of PBX3 reversed the inhibitory effect of butorphanol on proliferation, migration and invasion of breast cancer cells. Conclusions Butorphanol can reduce the proliferation, migration and invasion of breast cancer cells by inhibiting PBX3.

Key words: butorphanol, breast cancer, PBX3, proliferation, migration, invasion