关键词: 糖尿病心肌病, 心肌细胞肥大, 高通量测序, 长链非编码RNA

Abstract: Objective To explore the long-chain noncoding RNA (lncRNAs) expression and its possible biological functional analysis in hypotrophic cardiomyocytes induced by high glucose. Methods Neonatal mice cardiomyocytes were cultured by high glucose for processing 48 h and then randomly divided into the control group (Con) and the high sugar group (HG), the long-chain noncoding RNA (lncRNAs) expression were measured by using high throughput sequencing. Results 14 differentially expressed lncRNAs were screened, of which 5 were upregulated (ENSMUST00000195674, ENSMUST00000183707, ENSMUST00000195992, MSTRG.8385.2, MSTRG.9749.1) and 9 downregulated (ENSMUST00000195426, ENSMUST00000186944, ENSMUST00000182639, ENSMUST00000181094, ENSMUST00000125001, ENSMUST00000210380, MSTRG. 8302.1, MSTRG.11564.1, MSTRG.13750.1). ENSMUST00000182639, ENSMUST00000181094, ENSMUST00000195674, MSTRG.8302.1 may participate in PI3K-Akt signaling pathway, AMPK signaling pathway, mTOR signaling pathway, Rap1 signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Hypertrophic cardiomyopathy, cardiomyopathy, or Oxidative phosphorylation by regulating Igf1.Conclusions LncRNAs in hypertrophic cardiomyocytes induced by high glucose are differentially expressed and might participated in the pathophysiological processes of hyperglycemic cardiomyocyte hypertrophy through corresponding signaling pathways.

Key words: diabetic cardiomyopathy, cardiomyocytes hypotrophy, high-throughput sequencing , lncRNA