基础医学与临床 ›› 2019, Vol. 39 ›› Issue (3): 385-391.

• 临床研究 • 上一篇    下一篇

过表达miR-5195-3p对结直肠癌细胞凋亡的影响

张娟1,冯燕2,和水祥1   

  1. 1. 西安交通大学第一附属医院消化内科
    2. 新疆维吾尔自治区人民医院
  • 收稿日期:2018-02-13 修回日期:2018-06-27 出版日期:2019-03-05 发布日期:2019-03-05
  • 通讯作者: 和水祥 E-mail:heshuixiangxajd@163.com

Effect of miR-5195-3p over-expression on colorectal cancer cell apoptosis

  • Received:2018-02-13 Revised:2018-06-27 Online:2019-03-05 Published:2019-03-05

摘要: 目的 探讨miR-5195-3p对Runx相关转录因子3(RUNX3)的靶向调节作用及对结直肠癌细胞凋亡的影响。方法 RT-qPCR和Western blot分别检测结直肠癌组织与癌旁组织中miR-5195-3p和RUNX3蛋白的表达。将结直肠癌细胞SW480随机分为5组:对照组(Control)、miR-5195-3p mimic组、mimic control组、miR-5195-3p inhibitor组、inhibitor control组。Wetsern blot检测RUNX3的蛋白表达;流式细胞仪检测细胞凋亡。在细胞系中转染RUNX3过表达质粒后检测细胞凋亡。结果 与癌旁组织中相比,miR-5195-3p在结直肠癌组织中的表达显著升高,RUNX3的蛋白表达明显降低(P < 0.05)。过表达miR-5195-3p显著下调了SW480细胞中RUNX3的表达,并降低细胞凋亡水平(P < 0.05);抑制miR-5195-3p明显上调RUNX3的表达,并上调细胞凋亡水平(P < 0.05)。此外与miR-5195-3p mimic组相比,RUNX3过表达质粒的共转染组显著降低细胞凋亡水平(P < 0.05)。结论 MiR-5195-3p可能作为促癌基因在结直肠癌中发挥作用,其作用与靶向调节RUNX3相关。

关键词: 结直肠癌, RUNX3, miR-5195-3p, SW480, 细胞凋亡

Abstract: Objective To study the effect of miR-5195-3p on runt-related transcription factor 3 (RUNX3) and colorectal cancer cell apoptosis. Methods The mRNA expression of miR-5195-3p and protein expression of RUNX3 were detected by RT-qPCR and Western blot, respectively. The SW480 cells were randomly divided into 5 groups: control group, miR-5195-3p mimic group, mimic control group, miR-5195-3p inhibitor group and inhibitor control group. The expression level of RUNX3 was measured by western blot. Cell apoptosis was detected by flow cytometry. Results The expression of miR-5195-3p was significantly increased, while the protein expression of RUNX3 was dramatically decreased in colorectal cancer tissue compared with non-tumor normal tissue (P<0.05). Overexpression of miR-5195-3p significantly down-regulated RUNX3 expression and decreased cell apoptosis, while inhibition of miR-5195-3p significantly up-regulated RUNX3 expression and increased cell apoptosis in SW480 cells (P<0.05). Additionally, co-transfection with RUNX3 overexpression vector obviously elevated cell apoptosis compared with miR-5195-3p mimic group (P<0.05). Conclusions miR-5195-3p may act as a oncogene in colorectal cancer, and its function was related to the target regulation of RUNX3.

Key words: colorectal cancer, RUNX3, miR-5195-3p, SW480, cell apoptosis