基础医学与临床 ›› 2016, Vol. 36 ›› Issue (11): 1542-1547.

• 研究论文 • 上一篇    下一篇

欧前胡素通过提高自噬水平缓解大鼠雌激素缺乏性骨质疏松

谭亮,王小娇,肖炜   

  1. 解放军第十一医院
  • 收稿日期:2016-01-25 修回日期:2016-04-30 出版日期:2016-11-05 发布日期:2016-10-24
  • 通讯作者: 肖炜 E-mail:345565667@qq.com

Imperatorin ameliorates estrogen deficiency induced-osteoporosis through elevation of autophagy in rats

  • Received:2016-01-25 Revised:2016-04-30 Online:2016-11-05 Published:2016-10-24

摘要: 目的 探寻欧前胡素对大鼠雌激素缺乏性骨质疏松的疗效及机制。方法 将大鼠随机分为对照(control),OVX和OVX+Imp组。建模后10d,给OVX+Imp组、对照组和OVX组大鼠分别尾静脉注射欧前胡素75 mg/kg或氯仿溶剂,2次/周,10周。μCT和HE切片分析股骨远端骨小梁形态及参数。应力测试股骨及L5椎骨力学强度。钙黄绿标记内源性矿物沉积。ELISA检测血清PINP和B-ALP。ALP染色检测BMMSCs成骨分化能力。Real-time PCR和Western blot分别检测mRNA和蛋白表达。HE染色检测皮下移植物异位成骨。结果 1)OVX组骨量、骨小梁参数、股骨和L5椎骨力学强度、矿物沉积速率、血清PINP和B-ALP均低于对照组和OVX+Imp组(P<0.05)。2)OVX组内源性BMMSCs的ALP活性、成骨基因表达及异位成骨能力均低于对照组和OVX+Imp组(P<0.05)。3)OVX组内源性BMMSCs自噬水平低于对照组和OVX+Imp组(P<0.05)。4)BMMSCs+3-MA组自噬水平低于BMMSCs组,成骨能力相应降低(P<0.05);BMMSCs+3-MA+Imp组自噬水平高于BMMSCs+3-MA组,成骨活性相应升高(P<0.05)。结论 欧前胡素通过提高自噬水平恢复内源性BMMSCs成骨能力,以治疗雌激素缺乏性骨质疏松。

关键词: 欧前胡素, 骨质疏松, 骨髓间充质干细胞, 自噬, 成骨分化

Abstract: Objective To determine the therapeutic effect and mechanism of imperatorin on estrogen deficiency induced-osteoporosis. Methods Rats were randomly distributed into group control, OVX and OVX+Imp. Ten days after modeling, rats in group OVX+Imp,control and OVX were intravenously administrated with Imperatorin, 75 mg/kg or chloroform, twice per week, 10 weeks.Trabecular morphologyand indexes of distal femurs were analysed by μCT and HE staining. Mechanics strength of femurs and lumbars was analyzed by mechanical testing.Mineral deposition was labeling by calcein. Serum PINP and B-ALP were detected by ELISA. Ossification of BMMSCs was tested by ALP staining. mRNAs and proteins were tested by real-time PCR or Western blot respectively. New bone formation in subcutaneous implants was tested by HE staining. Results 1)Bone volume, trabecular indexes, mechanics strength of femurs and lumbars, mineral deposition, serum PINP and B-ALP of OVX were lower than control and OVX+Imp(P<0.05).2)ALP activity, osteogenic genes and ectopic osteogenesis of endogenous BMMSCs from OVX were lower than control andO VX+Imp(P<0.05).3) Autophagy of OVX was lower than control and OVX+Imp(P<0.05).4) Autophagy BMMSCs+3-MA was lower than BMMSCs, and the osteogenesis correspondly decreased(P<0.05);autophagy BMMSCs+3-MA+Imp was higher than BMMSCs+3-MA, and the osteogenesisc orrespondly increased(P<0.05).Conclusions Imperatorin ameliorated estrogen deficiency induced-osteoporosis through osteogenesis promotion caused by elevation of autophagy.

Key words: Imperatorin, osteoporosis, BMMSCs, autophagy, osteogenesis

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