基础医学与临床 ›› 2016, Vol. 36 ›› Issue (9): 1252-1256.

• 研究论文 • 上一篇    下一篇

RON基因在膀胱癌组织中的表达及对T24细胞增殖的影响

张莎莎,刘水,罗华铭,张翾   

  1. 重庆医科大学附属永川医院泌尿外科
  • 收稿日期:2015-10-08 修回日期:2016-01-03 出版日期:2016-09-05 发布日期:2016-08-30
  • 通讯作者: 张翾 E-mail:xuanzh168@126.com
  • 基金资助:
    重庆市卫计委资助项目

Expression of RON in bladder cancer tissues and its effect on proliferation in T24 cell line

  • Received:2015-10-08 Revised:2016-01-03 Online:2016-09-05 Published:2016-08-30

摘要: 目的 探讨RON基因对T24细胞增殖与迁移的影响。 方法 收集膀胱癌组织标本,通过RT-PCR检测RON在膀胱癌组织中的表达,其次用siRNA技术干扰T24细胞RON,用RT-PCR、western blot检测干扰效果,CCK-8、transwell实验及流式细胞术分别检测细胞的增殖、迁移及细胞凋亡。 结果 膀胱癌组织中RON表达与TNM分期呈正相关(P<0.05)。在T24细胞的RON-siRNA组,细胞增殖能力减弱(P<0.05),迁移与侵袭能力降低(P<0.05),凋亡细胞数增加(P<0.05),同时P-ERK蛋白表达减少,但Total-ERK表达不变。 结论 RON基因促进膀胱癌细胞的增殖与迁移,有望成为膀胱癌治疗的新靶点。

关键词: siRNA , 膀胱癌, T24细胞, 巨噬细胞刺激蛋白受体, RON

Abstract: Objective This study aimed to investigate effects of RON on proliferation and migration in T24 cell line. Methods First, RT-PCR was used to detect expression of RON mRNA in bladder cancer tissues. Second, Knockdown of RON expression in T24 cell line was conducted by transfection of a chemical synthesized small interfering RNA specifically targeted to RON. Efficacy of RON-silenced was determined ?by real time -PCR and western blot, The CCK-8, Transwell migration and invasion assays were performed to measure the proliferation, migration and invasion abilities, respectively. Flow cytometry was adopted to detect cell apoptosis. Results The results showed that the RON mRNA relative expression level was positive correlation with TNM stage; in RON-siRNA group, cell proliferation and migration abilities were degraded (P<0.05), and cell apoptosis was increased (P<0.05), compared with other groups. Conclusion RON gene promoted proliferation and migration of bladder cancer cells, maybe become a new target for the treatment of bladder cancer.

Key words: small interfering RNA, bladder cancer, T24 cells, MST1R, RON

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