熊果酸对THP-1细胞HMGB1表达和NF-кB活性的影响

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (9): 1257-1261.

熊果酸对THP-1细胞HMGB1表达和NF-кB活性的影响

孙爱平¹,刘小梅²,胡华¹,冯欢欢¹,张国俊¹,宋向凤¹

1. 新乡医学院
2. 河南理工大学医学院

收稿日期:2015-10-20 修回日期:2016-03-25 出版日期:2016-09-05 发布日期:2016-08-30
通讯作者: 宋向凤 E-mail:xfsong@xxmu.edu.cn
基金资助:
河南省教育厅科学技术研究重点项目;新乡医学院重点领域招标课题;新乡医学院高学历人才资助课题

Effects of ursolicacid on HMGB1 expression and NF-кB activity of THP-1 monocytes

Received:2015-10-20 Revised:2016-03-25 Online:2016-09-05 Published:2016-08-30

摘要/Abstract

摘要： 目的研究熊果酸（UA）对人单核细胞系THP-1细胞HMGB1表达和NF-кB活性的影响。方法用不同浓度的UA（0、0.1、1、5和10μmol/L）作用细胞后进行如下检测：1）MTT检测细胞增殖；2）RT-PCR法检测HMGB1和p65 mRNA表达；3）Western blot检测HMGB1和p65蛋白的表达；4）荧光素酶报告基因转染，质粒pNF-κB-luc瞬时转染THP-1细胞,转染40h后加1μmol/LUA作用,于转染48h后进行荧光素酶活性测定。结果 1）随着UA浓度的增高，可明显抑制THP-1细胞的增殖；2）不同浓度的UA对THP-1细胞HMGB1 mRNA和蛋白表达，呈现出一定的双向性，且以1 μmol/L UA作用最强（P<0.05）；3）UA可增强p65 mRNA和蛋白表达以及NF-κB活性。结论 UA可影响单核细胞HMGB1表达和NF-кB活性。

关键词: 熊果酸, THP-1细胞, 高迁移率族蛋白1, 核因子-кB

Abstract: Objective To investigate the effects of ursolic acid on the expression of HMGB1 and activity of NF-κB in THP-1 cells. Methods After being stimulated by different concentrations UA (0,0.1,1,5 and 10μmol/L), the cells were detected correspondingly.⑴The cell proliferation were checked by MTT. ⑵The HMGB1 and p65 destination genes were amplificated with PCR.⑶HMGB1 and p65 protein was detected by Western blot. ⑷Transfecting of Plasmid pGl3-NF-кB-luciferase report gene:Plasmid pGl3-NF-кB-luciferase report gene was transient transfected into THP-1 cells.After transfecting for 40 hours, 1 μmol/L UA were added. Luciferase activity detection was performed when transfecting for 48 hours. Results ⑴UA can obviously inhibit THP-1 cell proliferation with the concentration increasing.⑵The influence of UA on THP-1 cell HMGB1 mRNA and protein exhibits a certain bidirectional and the influence of 1 μmol/L UA is the strongest.⑶UA can influence the expression of p65 mRNA and protein andaffect the NF-кBactivity.Conclusion UA can influence the HMGB1 expression and NF-кB activity of THP-1 cell.

Key words: Ursolic acid, THP-1, HMGB1, NF-кB

中图分类号:

孙爱平刘小梅胡华冯欢欢张国俊宋向凤. 熊果酸对THP-1细胞HMGB1表达和NF-кB活性的影响[J]. 基础医学与临床, 2016, 36(9): 1257-1261.