关键词: Gli2, 结肠癌, 增殖, 侵袭

Abstract: bjective To explore the effect and mechanism of Gli2 on the proliferation,migration and invasion in colon cancer cell line SW480.Methods Complementary shRNA oligonucleotides targeting the Gli2 gene was designed and inserted into lentiviral vector. Then shRNA lentivirus were transfected into SW480 cells and then fluorescence photographs were taken. The gene silencing efficiency was measured by qRT-PCR and Western blot. Interfered group,negative group and blank group were set up.MTT,doubling time and colony-forming test were used to examine the proliferation of SW480.Migration and invasion ability were evaluated by Transwell chambers assay;Meanwhile,the qRT-PCR、Western blot were used to examine cyclinD1 and MMP-2 mRNA and protein expression. Results Green fluorescent protein was observed under fluorescence microscope after 72h in SW480 cells transfected with lentivirus.Compared with the negative control group and blank control group,the expression of Gli2 in the interfered group was lower,the cells grew slower,the doubling time was longer and the colony-forming ability was decreased,migration and invasion ability were weaken,meanwhile,the expression of cyclinD1 and MMP-2 was declined significantly(p<0.05). Conclusion Silencing Gli2 gene could suppress proliferation,migration and invasion ability in colon cancer cell line SW480.its mechanism may be related to the down-regulation of cyclinD1 and MMP-2.

Key words: Gli2, colon cancer, proliferation, invasion