关键词: 生物信息学, microRNA, hsa-miR-10b, 靶基因预测

Abstract: Objective To analyze the expression levels of hsa-miR-10b in three cervical cancer cell lines, and predict the target genes of hsa-miR-10b. Methods PCR was applied to measure expression levels of hsa-miR-10b in C-33A, HeLa and CaSki. The relative studies on hsa-miR-10b were retrieved from PubMed. The sequence and genome characteristics of hsa-miR-10b were analyzed on line by miRbase and NCBI. The target genes of hsa-miR-10b were predicted by TargetScan, PicTar and miRanda, and then demonstrated by Gene Ontology and Pathway Enrichment analysis. Results Compared with C-33A, the expression of hsa-miR-10b significantly reduced in HeLa and Caski (P<0.01). Current studies showed that　hsa-miR-10b was related with multiple tumorigenesis. Hsa-miR-10b located in human chromosome 2q31.1 and highly conserved among different species. The target genes were enriched in the biological processes of transcription, gene expression regulation, cell proliferation and etc.(P<0.05). Pathway analysis showed that these target genes were related to the signaling pathways of cancer, cell cycle, ARF and etc. (P<0.05). Conclusion Hsa-miR-10b may have extensive functions, and closely related with the occurrence and development in cervical cancer. Prediction of target genes provides a theoretical basis for the further study.

Key words: bioinformatics, microRNA, hsa-miR-10b, target gene prediction