关键词: Fcγ 受体, Fcε 受体, 免疫球蛋白G, 免疫球蛋白E, 过敏

Abstract: Objective To investigate the expression of Fcγ and ε receptor on mouse trigeminal ganglions and the expression change f neuronal Fcγ and ε receptor in allergy. Methods Allergy model in mouse was induced by intraperitoneal injection of OVA and Alum. ELISA was applied to identify the level of the serum total IgE in the na?ve and allergic mouse. Western blotting and immunofluorescence were employed to detect the expression and the change of Fcγ and ε receptor on na?ve and allergic mouse trigeminal ganglions. Results Fcγ and ε receptor were expressed on mouse trigeminal ganglions. The FcγRI, the IgG high-affinity activating receptor, and FcγRII that is the low-affinity inhibitory receptor FcγRII were only expressed on trigeminal ganglions neurons, but the IgG low-affinity activating receptor FcγRIII were only expressed on the satellite glial cells, not neurons of mouse trigeminal ganglions. The only IgE high-affinity activating receptor FcεRI was just expressed on trigeminal ganglions neurons, but the IgE low-affinity receptor FcεRII was expressed on neurons and satellite glial cells of trigeminal ganglions. Compare with na?ve mouse, the level of serum total IgE and the FcεRI and FcγRII protein of trigeminal ganglions were increased in allergic mouse. But the protein levels of trigeminal ganglions FcεRII and FcγRI were decreased in allergic mouse. The expression of FcγRIII was no significantly difference between na?ve mouse and allergic mouse trigeminal ganglions. Conclusion Fcγ and ε receptor on mouse trigeminal ganglions may involve in the allergic desease.

Key words: Fcγ receptor, Fcε receptor, IgG, IgE, Allergy