关键词: SKf525A, CYP2J3, 11,12-EET

Abstract: Objective: To observe the effect of Prodifen (SKf525A) on rat thoracic aorta artery stenosis after balloon angioplasty and discuss the relationship between endogenous Cytochrome P450/epoxyeicosatrienoic acids (CYP2J2/EETs) system and artery stenosis after vascular injury. Methods: The 24 Wistar rats were randomly divided into four groups assigned to the following treatments: Sham group, I group (treated by ballon angioplasty for 15d). Using Sham+Skf group (administrated with SKF525A), I+Skf group (administrated with SKF525A, then treated by ballon angioplasty for 15d). Relative luminal area (RLA) and the intimal proliferation index (IPI) were observed by HE staining; The expression of Cytochrome P450 epoxygenase 2J3 (CYP2J3) mRNA was determined by semi-quatative reverse transcription polymerase chain reaction (RT-PCR); the level of 11,12-EET was detected by high pressure liquid chromatography (HPLC). Results: Compared with Sham group, RLA was significantly decreased, IPI was significantly increased, CYP2J3 mRNA expression and the content of 11,12-EET were significantly increased in I group (P<0.01). Compared with I group, SKf525A treatment significantly decreased RLA, increased IPI, and decreased CYP2J3 mRNA expression and the content of 11,12-EET (P<0.01). Conclusions：CYP2J3/EETs system can inhibit stenosis after vascular injury.

Key words: SKf525A, CYP2J3, 11,12-EET