普罗地芬加重大鼠胸主动脉球囊成形术后动脉狭窄程度

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (1): 22-25.

普罗地芬加重大鼠胸主动脉球囊成形术后动脉狭窄程度

王晶,丁宁,王宇

首都医科大学附属北京同仁医院

收稿日期:2014-05-15 修回日期:2014-07-21 出版日期:2015-01-05 发布日期:2014-12-30
通讯作者: 王宇 E-mail:tryywy@163.com
基金资助:
北京市自然科学基金

Prodifen increased thoracic aorta artery stenosis after balloon angioplasty in rat

Received:2014-05-15 Revised:2014-07-21 Online:2015-01-05 Published:2014-12-30
Supported by:
Beijing Natural Science Foundation

摘要/Abstract

摘要： 目的观察普罗地芬（SKf525A）对大鼠胸主动脉球囊成形术后动脉狭窄程度的影响，探讨内源性细胞色素P450表氧化酶2J3（Cytochrome P450 epoxygenases，CYP2J3)/环氧-二十碳三烯酸(epoxyeicosatrienoic acids, EET)系统与血管损伤后动脉狭窄的关系。方法 Wistar雄性大鼠随机分为4组：假手术组(Sham组) ，胸主动脉球囊损伤组(I组)，用普罗地芬干预上述两组分别为： Sham+Skf及I+ Skf组。 HE染色观察胸主动脉相对管腔面积（RLA）及内膜增生指数(IPI)，用RT-PCR法检测胸主动脉CYP2J3 mRNA表达，用高效液相色谱分析法测定胸主动脉11,12-环氧-二十碳三烯酸(11,12-EET)含量。结果与Sham组相比， I组RLA明显缩小，IPI明显增高(P<0.01)， CYP2J3 mRNA的表达和11,12-EET含量均明显升高(P<0.01)；SKf525A处理后明显缩小I组RLA及明显增加胸主动脉IPI，而明显降低CYP2J3 mRNA的表达和11，12-EET含量(P<0.01)；结论 CYP2J3/ EETs系统具有拮抗血管损伤后狭窄的作用。

关键词: SKf525A, CYP2J3, 11,12-EET

Abstract: Objective: To observe the effect of Prodifen (SKf525A) on rat thoracic aorta artery stenosis after balloon angioplasty and discuss the relationship between endogenous Cytochrome P450/epoxyeicosatrienoic acids (CYP2J2/EETs) system and artery stenosis after vascular injury. Methods: The 24 Wistar rats were randomly divided into four groups assigned to the following treatments: Sham group, I group (treated by ballon angioplasty for 15d). Using Sham+Skf group (administrated with SKF525A), I+Skf group (administrated with SKF525A, then treated by ballon angioplasty for 15d). Relative luminal area (RLA) and the intimal proliferation index (IPI) were observed by HE staining; The expression of Cytochrome P450 epoxygenase 2J3 (CYP2J3) mRNA was determined by semi-quatative reverse transcription polymerase chain reaction (RT-PCR); the level of 11,12-EET was detected by high pressure liquid chromatography (HPLC). Results: Compared with Sham group, RLA was significantly decreased, IPI was significantly increased, CYP2J3 mRNA expression and the content of 11,12-EET were significantly increased in I group (P<0.01). Compared with I group, SKf525A treatment significantly decreased RLA, increased IPI, and decreased CYP2J3 mRNA expression and the content of 11,12-EET (P<0.01). Conclusions：CYP2J3/EETs system can inhibit stenosis after vascular injury.

Key words: SKf525A, CYP2J3, 11,12-EET

中图分类号:

R543.1

王晶丁宁王宇. 普罗地芬加重大鼠胸主动脉球囊成形术后动脉狭窄程度[J]. 基础医学与临床, 2015, 35(1): 22-25.