关键词: 维持性血液透析, 高磷血症, 微炎症, 氧化应激

Abstract: Objective To investigate the effect of high phosphate on mRNA and protein expression of intracellular NF-κB and NOX2/gp91 in PBMC of MHD patients and serum inflammation mediators and MDA production，so as to understand the possible relationship among hyperphosphatemia, micro-inflammatory states and oxidative stress. Method They were divided into five groups: control, MHD, dose, time and blank groups.Western blot and Real-time PCR were respectively used to determine the mRNA and protein expression of NF-κBP65、NOX2.The concentration of IL-6 in the culture supernatant was quantified by the ELISA kit and the TBA assay kit．Results NF-κB and NOX2 signal pathway in PBMCs show abnormal activation status in MHD patients. Both expression of NOX2/gp91 mRNA and protein levels increased dose-and time-dependent manners totally. IL-6 and MDA production in supernatant up-regulate dose-and time-dependent manners totally. NF-κB P65 mRNA and Phospho-NF-κBP65 protein were positively correlated with IL-6 production. NOX2/gp91 mRNA and protein expression were positively correlated with MDA production. Conclusion High phosphate could increase the production of supernatant IL-6 and MDA by activating NF-κB and NOX2 signaling pathway in PBMCs, thus could be a risk factor for microinflammatory and oxidative stress status of MHD patients.

Key words: maintenance hemodialysis, hyperphosphatemia, microinflammatory, oxidative stress