关键词: 宫颈癌, NF-κB, miRNA-15b, miRNA-16

Abstract: Objective To investigate the expression of miR-15b and miR-16 as well as the status of NF-κB signaling in xenografted cervical cancer and its adjacent tissues，which will provide new insight for the diagnosis and treatment of cervical cancer. Methods The xenograft mouse model of cervical cancer was successfully established. Xenografted cancer and its adjacent tissues, normal fat tissues were collected respectively. The expression of miR-15b and miR-16 were detected by real-time PCR. The expression and sublocation of NF-κB(P 65) was analyzed by immunohistochemistry technology. Results HE staining of the xenograft indicated that the model is successful. The miR-15b levels in the cancer are 32.21±3.67, which significant higher than its adjacent tissues25.16±1.86 and normal fat tissue 1.00±0.12 (P<0.05)，adjacent tissues also significant higher than normal fat tissue (P<0.05); miR-16 levels in the cancer are 28.63±2.34,which significant higher than adjacent tissues22.16±1.76 and normal fat tissue 1.00±0.12 (P<0.05)，adjacent tissues also significant higher than normal fat tissue (P<0.05). NF-κB(P 65) was mainly localized in cytosol of normal adipose tissues，whereas in cervical cancer tissues and adjacent non-tumorous tissues，NF-κB(P 65) translocated into the nucleus. Conclusion NF-κB signal pathway and its regulated miR-15b and miR-16 were upregulated significantly in both cancer tissues and its adjacent tissues. The NF-κB signal pathway and its regulated miR-15b and miR-16 were involved in early molecular events of cervical cancer.

Key words: cervical cancer, NF-κB, miRNA-15b, miRNA-16