关键词: 关键词：表皮生长因子, 人羊膜间充质干细胞, 基因表达谱, 测序, 生物信息学

Abstract: Objective To characterize the effect of epidermal growth factor on the spectrum of gene expression in the cultured human amnion mesenchymal stem cells (hAMSCs), and explore the underlying molecular mechanism. Methods Illumina RNA-Seq was used to detect the alteration in the gene expression profiles induced by 100 ng/ml epidermal growth factor in hAMSCs. Real-time PCR was employed to verify the results of selected differentially expressed genes. Results A total of 104 differential genes were found（FDR≤0.001 and |log2Ratio|≥1）in human amnion mesenchymal stem cells after treatment with epidermal growth factor for 24h, including 56 up-regulated and 48 down-regulated ones. GO enrichment of biological process analysis 26 biological processes have significant（P≤0.05）；KEGG signaling pathway enrichment analysis found that significant interaction between cytokines and cytokine signaling pathway is significant（Q≤0.05）.Conclusion EGF intervention hAMSCs changes in gene expression profile, Significantly differentially expressed genes and signaling pathways plays an important role in hAMSCs proliferation and migration and wound healing in the process.

Key words: Key words: epidermal growth factor, human amniotic mesenchymal stem cells, gene expression profile, sequencing , bioinformatics