关键词: 脂肪, 间充质干细胞, 创伤愈合

Abstract: Objective To explore the effects of adipose derived mesenchymal stem cells（hADSCs）on cutaneous wound healing and provide a new method for clinical skin repair in the future. Methods The hADSCs were isolated by mechanical separation and subsequent enzymatic digestion. The expression profiles of CD90,CD105，CD34andCD45 were examined by immunofluorescence. The hADSCs from passage 3-5 were used for bone, osteogenic, adipogenic differentiation assays by culturing in appropriate induction medium.The mice were anesthetized and a 12-cm full-thickness excisional skin wound was made on the back of each mouse. After labeling with superparamagnetic iron oxide nanoparticles (SPIONs ), hADSCs were injected intradermally around the wound at four different injection sites. After o,7and 14d , the area of the wounds were measured and performed to evaluate the quality of wound healing compare with control. Results The morphological feature of hADSCs displayed fibroblast-like phenotype.The cells were positive for stem cells markers，including CD90 and CD105，and negative for CD34 and CD45,as shown by immunofluorescence. Osteogenic,chondrogenic, adipogenic differentiations were revealed in hADSCs when cultured in specific medium．The first 3 days of the experimental mice in each group after skin wound, wound gradually reduced, most of the wounds healed after 21d. Compared with the control group （26.7%±1.9）at 7d and（47.3%±2.3）at 14d，, the most obvious effect at 7d （74.6%±4.1）and 14d（96.5%±1.47）after transplantation（P＜0.05）. Conclusion hADMSCs transplantation can improve wound healing.

Key words: adipose, mesenchymal stem cells, wound healing