关键词: 福辛普利, 人肾小管上皮细胞, 转化生长因子β激活激酶, 肾间质纤维化

Abstract: Abstract：Objective To investigate the effects of fosinopril(FOS) on expression of TAK1 and proliferation of the human renal tubular epithelial cells induced by LPS. Methods Human renal tubular epithelial (HK-2) cells were divided into three groups: blank control group(Ctol), LPS group (10 μg/L), FOS group (LPS 10 μg/L+ FOS 1×106 mol/L). At 12, 24, 48 hours, HK-2 proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The change of fibronectin (FN) in the supernatants of the cultured HK-2 was detected by enzymelinked immunosorbent assay (ELISA). The protein expressions of TAK1 and FN were measured by Western blot. The mRNA expressions of TAK1 was measured by real-time quantitative PCR. Results The cell proliferation and the expression of FN were increased, and the expressions of protein and mRNA of TAK1 in LPS group were upregulating significantly compared with control group from 12 h (P<0.01，P<0.05), but they were downregulating in FOS group compared with LPS group(P<0.01). Conclusion FOS probably delay renal interstitial fibrosis by inhibiting proliferation and activation of HK-2 and decreasing accumulation of extracellular matrix.

Key words: Fosinopril, Human renal tubular epithelial cells, TGFβ-activated kinase 1, Renal interstitial fibrosis