关键词: 磁性纳米粒子-抗体复合物, 密度梯度离心, 线粒体, 分离纯化

Abstract: Abstract: Objective Studying differences between magnetic nanoparticles-antibody complex and the traditional method to isolate mitochondria from human liver cancer tissues. Methods Using magnetic nanoparticles-antibody complex and density gradient centrifugation to isolate mitochondria of human liver cancer tissues separately, then scanning electron microscopy was used to scan the morphology of mitochondria, Western blot was used to detect mitochondrial protein markers and flow cytometry was used to analyze the alteration of mitochondrial membrane potential. Results The mitochondria isolated by magnetic nanoparticle-antibody had intergral structure, contained almost membrane no contaminant of nucleus, cell membrane, peroxisomes and lysosomes. The mitochondrial potential was stable. Conclusion Comparing with the traditional method, magnetic nanoparticle-antibody complex method gets more advantages, such as the preparation is easier, the structure of mitochondria is intact, the purity and activity of mitochondria are higher, large equipments are not needed and so on.

Key words: Magnetic nanoparticles-antibody complex, Density gradient centrifugation, Mitochondria, Purification