基础医学与临床 ›› 2011, Vol. 31 ›› Issue (7): 728-735.

• 研究论文 • 上一篇    下一篇

头颈肿瘤浸润gdT细胞的数量及表型与临床分期相关

殷珊珊1,陈兴明2,3,康宁4,陈晓巍5,高志强5,何维1   

  1. 1. 中国医学科学院基础医学研究所 北京协和医学院基础学院
    2.
    3. 中国医学科学院北京协和医院
    4. 中国医学科学院 基础医学研究所 北京协和医学院 基础学院
    5. 中国医学科学院 北京协和医学院 北京协和医院耳鼻喉科
  • 收稿日期:2010-11-11 修回日期:2010-12-01 出版日期:2011-07-05 发布日期:2011-07-05
  • 通讯作者: 康宁 E-mail:n.kang@126.com
  • 基金资助:
    国家重点基础研究发展计划;中国医学科学院基础医学研究所-北京协和医院“基础与临床联合攻关项目”

Quantities and phenotypes of tumor infiltrating gdT cells are related to clinical staging of head and neck tumor

Shan-shan YIN1,Xing-ming CHEN2,2,Ning KANG1,Xiao-wei CHEN2,Zhi-qiang GAO2,Wei HE1   

  1. 1. Institute of Basic Medical Sciences, CAMS & PUMC
    2.
  • Received:2010-11-11 Revised:2010-12-01 Online:2011-07-05 Published:2011-07-05
  • Contact: Ning KANG E-mail:n.kang@126.com

摘要: 目的 研究头颈肿瘤中浸润的Foxp3+gdT细胞的数量与头颈肿瘤临床分期的关系。方法 用组织研磨法分离患者肿瘤浸润淋巴细胞(TILs),用密度梯度离心法分离患者外周血单个核细胞(PBMCs);用固相化anti-pan-TCRgd单克隆抗体对TIL和PBMC进行2~4周的体外扩增,免疫荧光染色和流式细胞仪测定扩增前后的细胞;对头颈肿瘤组织芯片进行免疫组织化学染色。结果 15例头颈肿瘤组织标本TIL中gdT占CD3+T细胞的0.37%~12.35%,其中0.13%~34.38%为Foxp3+ gdT。扩增后TIL中gdT占CD3+T细胞的20.38%~82.87%,且以Vd1亚型为主。肿瘤组织芯片按照肿瘤TNM分期中的T分期分为4组,Foxp3+gdT细胞的比例随肿瘤分期的提高而增加。结论 现有证据证明了头颈肿瘤组织中存在Foxp3+ gdT细胞的浸润,并提示肿瘤组织中调节表型gdT细胞的数量可能与头颈肿瘤的预后相关。

关键词: 头颈肿瘤, gdT细胞, Foxp3, TNM分期

Abstract: Objective This study was designed to explore the relationship between infiltrated Foxp3+gdT cells and the clinical stage of head and neck tumor. Methods Head and neck tumor biopsies were mechanically minced to get tumor infiltrating lymphocytes (TILs). Peripheral blood mononuclear cells (PBMCs) from the patients were isolated by density gradient centrifugation. TILs and PBMCs were stimulated by immobilized anti-pan-TCRgd in vitro for two to four weeks. Freshly isolated and expanded cells underwent immunofluorescence staining and flowcytometry analysis. Commercial head and neck tumor tissue chips were used for immunohistochemical staining. Results Immunofluorescence staining of TILs freshly isolated from 15 head and neck tumor samples showed the gdT/CD3+T ratio as 0.37%~12.35%, Foxp3+ gdT/gdT ratio as 0.13%~34.38%. Following in vitro expansion, gdT/CD3+T cell ratio increased to 20.38%~82.87%. Expanded cells primarily belonged to the Vd1 subset. Tumor tissue chips were divided into four groups according to the T stage of the clinical TNM staging system. Subsequent immunohistochemical results indicated a correlation between the quantity of Foxp3+gdT cells and tumor TNM stage. Conclusion Our data demonstrate the existence of Foxp3+ gdT cells in the head and neck tumor tissue, and suggest that the quantity of tumor infiltrating gdT regulatory cells might be associated with the prognosis of head and neck tumor.

Key words: Head and neck tumor, gdT cell, Foxp3, TNM stage

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