基础医学与临床 ›› 2011, Vol. 31 ›› Issue (7): 736-739.

• 研究论文 • 上一篇    下一篇

细胞毒T淋巴细胞相关抗原4基因多态性与吸毒并HIV感染相关

刘莹1,刁波1,申元英2,周芳3,冯勇1,罗凡1,侯炜4   

  1. 1. 武汉大学医学院病毒学研究所
    2. .大理学院公共卫生学院
    3. 广州军区武汉总医院
    4.
  • 收稿日期:2010-12-01 修回日期:2010-12-28 出版日期:2011-07-05 发布日期:2011-07-05
  • 通讯作者: 侯炜 E-mail:houweidavid@yahoo.com.cn
  • 基金资助:
    国家重点基础研究发展计划

Association between cytotoxic T lymphocyte-associated antigen 4 gene haplotype and Drug users with HIV Infection

Ying LIU1,Bo DIAO2,Yuan-ying SHEN2,Fang ZHOU2,Yong FENG2,Fan LUO2,Wei HOU1   

  1. 1. Institute of Virology, School of Medicine, Wuhan University
    2.
  • Received:2010-12-01 Revised:2010-12-28 Online:2011-07-05 Published:2011-07-05
  • Contact: Wei HOU E-mail:houweidavid@yahoo.com.cn

摘要: 目的 研究细胞毒T淋巴细胞相关抗原4(CTLA-4)基因外显子1的49位点A/G和启动子-318位点C/T多态性与吸毒并HIV感染的相关性。方法 采用序列特异性引物聚合酶链反应 (PCR-SSP)方法,检测24例单纯吸毒者、41例吸毒合并HIV感染患者以及204例正常对照者CTLA-4基因外显子1的49位点A/G和启动子-318位点C/T的基因型。结果 与正常对照组比较,吸毒组C/C(12.50% VS 51.47%)(P<0.01)型频率明显下降;在吸毒合并HIV感染患者组,CTLA-4 A+49G基因型差异无统计学意义(P>0.05);CTLA-4 C-318T基因型差异有统计学意义(P<0.01),C/T(68.3% VS 45.6%) 、T/T(21.95% VS 2.94%)型频率明显上升,C/C(9.76% VS 51.47%)型频率显著下降。与吸毒组比较,吸毒合并HIV感染组中CTLA-4 A+49G及CTLA-4 C-318T各基因型频率变化无统计学差异(p>0.05)。结论 吸毒合并HIV感染与CTLA-4基因动态密切相关,C-318T基因型C/T、T/T型与吸毒合并HIV感染呈正相关,C/C行呈负相关。

关键词: 吸毒并HIV感染, 细胞毒T淋巴细胞相关抗原4, 基因多态性, 基因型

Abstract: Objective:To investigate the association of gene polymorphism of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) with drug users with HIV infection.Methods The A+49G transition polymorphism at position 49 (exon 1) and C-318T transition polymorphism at position 318 in promoter of the CTLA-4 gene were determined by polymerase chain reaction with sequence specific primers (PCR-SSP) method in 24 drug users,41 drug users with HIV Infection patients and 204 healthy controls.Results Compared with control group, in drug users group the frequency of C/C(12.50% VS 51.47%)( P<0.01) significantly reduced; in drug users with HIV infection group,no significant differences in the distribution of genotype and allele frequencies were observed in A+49G gene polymorphisms (p>0.05); however,there are significant differences in C-318T gene polymorphisms in drug users with HIV infection group(p<0.01),the frequency of C/T (68.3% VS 45.6%) and T/T(21.95% VS 2.94%)increased significantly,in contrast in C/C(9.76% VS 51.47%)significantly reduced. Compared with drug users group, no significant differences in the distribution of genotype and allele frequencies were observed in A+49G and C-318T gene polymorphisms (p>0.05) in drug users with HIV infection group.Conclusion There is close associativity between CTLA-4 Alleles and drug taking with HIV infection,in C-318T genotype, C/T and T/T were positively correlated with occurence of drug taking with HIV infection, but C/C was negatively correlated with it.

Key words: drug users with HIV infection , Cytotoxic T lymphocyte-associated antigen, Gene polymorphism, genotype