新生儿呼吸窘迫综合征患儿脐血TLR4、MIF、IL-1β水平及与预后的关系

doi:10.16352/j.issn.1001-6325.2026.01.0109

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (1): 109-113.doi: 10.16352/j.issn.1001-6325.2026.01.0109

新生儿呼吸窘迫综合征患儿脐血TLR4、MIF、IL-1β水平及与预后的关系

曲海新^1*, 梁俊霞¹, 袁二伟¹, 高鸿博², 隗晓华², 郭卫平²

河北北方学院附属第一医院 1.新生儿科;
2.儿科,河北张家口 075000

收稿日期:2024-11-27 修回日期:2025-02-26 出版日期:2026-01-05 发布日期:2025-12-29
通讯作者: ^*qorep06@163.com
基金资助:
河北省2023年度医学科学研究课题(20231448)

Levels of TLR4, MIF, IL-1β in umbilical cord blood of children with neonatal respiratory distress syndrome and their relationship with prognosis

QU Haixin^1*, LIANG Junxia¹, YUAN Erwei¹, GAO Hongbo², KUI Xiaohua², GUO Weiping²

1. Department of Neonatology;
2. Department of Pediatrics, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China

Received:2024-11-27 Revised:2025-02-26 Online:2026-01-05 Published:2025-12-29
Contact: ^*qorep06@163.com

摘要/Abstract

摘要： 目的通过检测脐血Toll样受体4(TLR4)、巨噬细胞迁移抑制因子(MIF)、白细胞介素-1β(IL-1β)水平,旨在分析三者与新生儿呼吸窘迫综合征(NRDS)患儿预后的关系。方法选取2023年1月至2024年6月期间206例早产儿作为研究对象,依据NRDS诊断标准分为NRDS组(n=120)和非NRDS组(n=86),根据NRDS患儿预后情况分为良好预后组(n=90)、不良预后组(n=30)。采用ELISA检测脐血TLR4、IL-1β、MIF表达水平。多因素Logistic回归分析影响NRDS患儿预后不良的因素,受试者工作特征(ROC)曲线分析脐血TLR4、MIF、IL-1β在预测NRDS患儿不良预后的效能。结果相较于非NRDS组,NRDS组脐血TLR4、MIF、IL-1β水平均明显升高(P＜0.05);相较于良好预后组,不良预后组脐血TLR4、MIF、IL-1β水平明显升高(P＜0.05);脐血TLR4、MIF、IL-1β水平升高均是影响NRDS患儿预后不良的危险因素(P＜0.05);脐血TLR4、MIF、IL-1β及三者联合预测NRDS患儿预后的AUC分别为0.843、0.845、0.850、0.976,三者联合预测效能显著优于单一指标(Z_{三者联合-TLR4}=3.841、Z_{三者联合-MIF}=3.615、Z_{三者联合-IL-1β}=2.452,P＜0.001、0.001、0.05)。结论 NRDS患儿脐血TLR4、MIF、IL-1β水平均显著升高,三者水平变化与NRDS患儿预后密切相关,且三者联合在预测NRDS患儿不良预后方面展现出更高的效能。

关键词: Toll样受体4, 巨噬细胞迁移抑制因子, 白细胞介素-1β, 新生儿呼吸窘迫综合征, 预后

Abstract: Objective To detect the expression level of Toll-like receptor 4 (TLR4), macrophage migration inhibitory factor (MIF) and interleukin-1β (IL-1β) in umbilical cord blood, and to analyze their relationship with the prognosis of neonatal respiratory distress syndrome (NRDS). Methods From January 2023 to June 2024, 206 premature infants in our hospital were reviewed. Complying with the NRDS diagnostic criteria, they were assigned into NRDS group (n=120) and non-NRDS group (n=86). Complying with the prognosis of NRDS patients, they were assigned into a good prognosis group (n=90) and a poor prognosis group (n=30). ELISA method was applied to detect the expression levels of TLR4, IL-1β, and MIF in umbilical cord blood. Multivariate Logistic regression was applied to analyze the factors affecting the prognosis of NRDS. Receiver operating characteristic (ROC) curve was applied to analyze the efficacy of cord blood TLR4, MIF, and IL-1β in predicting poor prognosis in children with NRDS. Results Compared with the non-NRDS group, the level of TLR4, MIF and IL-1β in umbilical cord blood were significantly higher in the NRDS group (P＜0.05). Compared with the good prognosis group, the levels of TLR4, MIF and IL-1β in umbilical cord blood were greatly higher in the poor prognosis group (P＜0.05). Elevated levels of TLR4, MIF, and IL-1β in umbilical cord blood was a group of risk factors affecting for poor prognosis in children with NRDS (P＜0.05). The AUC of TLR4, MIF, IL-1β, and their combination in predicting the prognosis of NRDS children in umbilical cord blood was 0.843, 0.845, 0.850, and 0.976, respectively. The effectiveness of the combined prediction of the three indicators was greatly better than that of a single indicator (Z_{combination-TLR4}=3.841,Z_{combination-MIF}=3.615, Z_{combination-IL-1β}=2.452, P＜0.001, 0.001, 0.05). Conclusions The level of TLR4, MIF, and IL-1β in umbilical cord blood of children with NRDS is greatly elevated. The changes in their level of these factors are closely related to the prognosis of NRDS children. The combination of the three shows higher efficacy in predicting poor prognosis in NRDS children.

Key words: Toll-like receptor 4, macrophage migration inhibitory factor, interleukin-1β, neonatal respiratory distress syndrome, prognosis

中图分类号:

R722.1

曲海新, 梁俊霞, 袁二伟, 高鸿博, 隗晓华, 郭卫平. 新生儿呼吸窘迫综合征患儿脐血TLR4、MIF、IL-1β水平及与预后的关系[J]. 基础医学与临床, 2026, 46(1): 109-113.

QU Haixin, LIANG Junxia, YUAN Erwei, GAO Hongbo, KUI Xiaohua, GUO Weiping. Levels of TLR4, MIF, IL-1β in umbilical cord blood of children with neonatal respiratory distress syndrome and their relationship with prognosis[J]. Basic & Clinical Medicine, 2026, 46(1): 109-113.

参考文献

[1]De Luca D, Tingay DG, van Kaam AH, et al. Epidemiology of neonatal acute respiratory distress syndrome: prospective, multicenter, international cohort study[J]. Pediatr Crit Care Med, 2022, 23:524-534.
[2]Hong H, Fu B, Wang R. Clinical value of serum miR-92 and miR-122 expression level combined with pulmonary ultrasound score in the prognosis of neonatal acute respiratory distress syndrome[J]. J Clin Lab Anal, 2022, 36:e24485-e24496.doi:10.1002/jcla.24485.
[3]Nucera F, Lo BF, Shen SS, et al. Role of atypical chemokines and chemokine receptors pathways in the pathogenesis of COPD[J]. Curr Med Chem, 2021, 28:2577-2653.
[4]Christopoulou ME, Aletras AJ, Papakonstantinou E, et al. WISP1 and macrophage migration inhibitory factor in respiratory inflammation: novel insights and therapeutic potentials for asthma and COPD[J]. Int J Mol Sci, 2024, 25:10049-10080.
[5]宋婷阁, 张鹏举, 耿彪, 等. 新生儿急性呼吸窘迫综合征患者血清miR-183-5p的表达及与IL-1β,IL-6和TNF-α水平的相关性[J]. 现代检验医学杂志,2021,36:55-68.
[6]匡凤梧, 许峰. 新生儿呼吸窘迫综合征的诊断和治疗[J]. 中国实用妇科与产科杂志, 2003, 19:335-337.
[7]Sweet DG, Carnielli VP, Greisen G, et al. European consensus guidelines on the management of respiratory distress syndrome: 2022 Update[J]. Neonatology, 2023, 120:3-23.
[8]张雨茜, 王荣花, 陈祥, 等. TLR4信号通路介导病毒性急性肺损伤与ARDS的研究进展[J]. 病毒学报, 2021, 37:1234-1243.
[9]Jalce G, Guignabert C. Multiple roles of macrophage migration inhibitory factor in pulmonary hypertension[J]. Am J Physiol Lung Cell Mol Physiol, 2020, 318:L1-L9.
[10]Bayraktar S, Tanyeri BB, Kihliç Ü. Umbilical cord levels of macrophage migration inhibitory factor in neonatal respiratory distress syndrome[J]. Turk J Med Sci, 2021, 51:722-726.
[11]Li X, Liu H. Expression and prognostic value of MIP-1α in neonatal acute respiratory distress syndrome[J]. Am J Transl Res, 2022, 14:7889-7897.
[12]Rong W, Liu C, Li X, et al. Caspase-8 promotes pulmonary hypertension by activating macrophage-associated inflammation and IL-1β (Interleukin 1β) production[J]. Arterioscler Thromb Vasc Biol, 2022, 42:613-631.

[1]	孙迎春, 李建军, 杨再波, 闫俊慧, 刘育硕. IgA肾病患者血清CHI3L1和sFlt-1水平与病情严重程度及预后相关[J]. 基础医学与临床, 2026, 46(1): 22-27.
[2]	薛雅馨, 陈安琪, 郑懿, 蓝云云. 联合检测血清sST2、GFR和SI对急性失代偿性心力衰竭患者预后的预测[J]. 基础医学与临床, 2025, 45(9): 1184-1189.
[3]	吴海丰, 李小龙, 李芳, 陈晓华, 宋瑞, 韩雪. 直肠癌组织中KIF23的表达与预后相关[J]. 基础医学与临床, 2025, 45(8): 1054-1058.
[4]	李楠, 张贵涛, 王彬, 冯瑶, 李淑娟, 周瑛华. 炎性反应相关指标与院内症状性卒中患者不良预后相关[J]. 基础医学与临床, 2025, 45(8): 1083-1087.
[5]	聂牧文, 孙昭, 李宁宁, 赵林, 白春梅. 原发性心包间皮瘤的临床特征与预后[J]. 基础医学与临床, 2025, 45(2): 222-228.
[6]	彭定越, 王玮婧, 张为, 杨乙, 包卓华, 吕渊, 李淑娟. 急性胼胝体梗死患者的临床特征和预后分析[J]. 基础医学与临床, 2025, 45(2): 234-238.
[7]	陈心如, 汤继宏, 肖潇, 武银银, 徐欢, 冯隽. 急性淋巴细胞白血病并发脑出血患儿的临床特点和预后[J]. 基础医学与临床, 2025, 45(11): 1480-1484.
[8]	李尔萃, 李伟之, 韩宁, 陈思, 范慧娟. 原儿茶酸减轻溃疡性结肠炎大鼠的结肠损伤[J]. 基础医学与临床, 2025, 45(10): 1306-1312.
[9]	黄航栋, 厉丹瑜, 朱晓锋. 血清PKM2、Gal-3、CitH3水平升高可预测机械通气并发肺部感染[J]. 基础医学与临床, 2025, 45(1): 86-90.
[10]	李顺乐, 李蓉, 田亚亚, 冉小丽, 赵延培, 徐蒙. 血清KLF5和actinin-4水平与肝细胞肝癌肝动脉化疗栓塞术治疗预后的关系[J]. 基础医学与临床, 2024, 44(9): 1284-1289.
[11]	侯楠, 刘源, 高俊, 王晶, 袁萌. 全血NPM1、MCP-1和肠道菌群与胃癌进展及预后相关[J]. 基础医学与临床, 2024, 44(8): 1137-1142.
[12]	操辰新, 唐辉, 耿瑞璇, 郭伏平, 白春梅, 王颖轶, 李太生. 循环CD4⁺CD45RA⁺CD62L⁺ T细胞与接受EGFR-TKI治疗的转移性非小细胞肺癌预后相关[J]. 基础医学与临床, 2024, 44(5): 658-664.
[13]	丁飞, 王洒, 黄常新. 非小细胞肺癌患者血清CA211水平和NK细胞数与预后相关[J]. 基础医学与临床, 2024, 44(2): 180-184.
[14]	张伟, 张振, 杨连任, 梁伟. 肺癌7项抗体水平与NSCLC患者化疗效果及预后的关系[J]. 基础医学与临床, 2024, 44(10): 1383-1387.
[15]	周昕羽, 汤继宏, 肖潇, 冯隽, 杨乐天, 徐欢, 武银银. 儿童急性B淋巴细胞白血病合并化疗诱导周围神经病变的临床特点及预后分析[J]. 基础医学与临床, 2024, 44(10): 1394-1399.

新生儿呼吸窘迫综合征患儿脐血TLR4、MIF、IL-1β水平及与预后的关系

Levels of TLR4, MIF, IL-1β in umbilical cord blood of children with neonatal respiratory distress syndrome and their relationship with prognosis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价