异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛

doi:10.16352/j.issn.1001-6325.2025.08.1028

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (8): 1028-1033.doi: 10.16352/j.issn.1001-6325.2025.08.1028

异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛

高彦东, 边步荣, 王波^*

榆林市第一医院(延安大学第二附属医院)手术麻醉科,陕西榆林 719000

收稿日期:2024-09-02 修回日期:2024-11-22 出版日期:2025-08-05 发布日期:2025-07-11
通讯作者: ^*27649843@qq.com
基金资助:
陕西省自然科学基础研究计划项目(2019JQ-983)

Propofol alleviates neuropathic pain induced by spinal nerve ligation in rats

GAO Yandong, BIAN Burong, WANG Bo^*

Department of Surgical Anesthesia, the First Hospital of Yulin(the Second Affiliated Hospital of Yan'an University), Yulin 719000, China

Received:2024-09-02 Revised:2024-11-22 Online:2025-08-05 Published:2025-07-11
Contact: ^*27649843@qq.com

摘要/Abstract

摘要： 目的探究异丙酚对脊神经结扎(SNL)构建的神经病理性疼痛(NP)模型大鼠的作用及机制。方法建立SNL大鼠模型,并将造模成功的30只大鼠随机分为模型(SNL)组、异丙酚干预模型(异丙酚＋SNL)组、异丙酚＋7-nitroindazole(N1)干预模型(异丙酚＋7-N1＋SNL)组(每组10只),另设假手术组(10只)。药物干预后:采用Von Frey法检测大鼠机械痛敏;热爪足底镇痛仪检测热缩足反射潜伏期;ELISA检测大鼠脊髓中白介素-6(IL-6)、γ干扰素(IFN-γ)水平;RT-qPCR检测脊髓背角中细胞因子信号转导抑制因子3(SOCS3)、精氨酸酶-1(Arg-1)、白细胞分化抗原68(CD68) mRNA表达;Western blot检测一氧化氮/环磷酸鸟苷(NO/cGMP)信号通路相关蛋白质表达水平。结果与假手术组相比,SNL组大鼠机械缩足反射阈值、热缩足反射潜伏期及Arg-1 mRNA和内皮型一氧化氮合酶(eNOS)、可溶性鸟苷酸环化酶(sGCa)蛋白表达水平均显著降低(P＜0.05),IL-6、IFN-γ水平、SOCS3、CD68 mRNA和诱导型一氧化氮合酶(iNOS)蛋白表达水平均显著增加(P＜0.05);与SNL组相比,异丙酚＋SNL组大鼠机械缩足反射阈值、热缩足反射潜伏期及Arg-1 mRNA和eNOS、sGCa蛋白表达水平均显著升高(P＜0.05),IL-6、IFN-γ水平、SOCS3、CD68 mRNA和iNOS蛋白表达水平均降低(P＜0.05);NO/cGMP信号通路抑制剂7-N1可逆转异丙酚对SNL大鼠的改善作用(P＜0.05)。结论异丙酚可能通过激活NO/cGMP信号通路,改善SNL大鼠的神经病理性疼痛症状。

关键词: 异丙酚, 脊神经结扎, 神经病理性疼痛, NO/cGMP信号通路

Abstract: Objective To evaluate the improvement effect and mechanism of propofol on neuropathic pain(NP) model rats induced by spinal nerve ligation(SNL). Methods An SNL rat model was established, and 30 successfully modeled rats were randomly divided into SNL group, propofol+SNL group and propofol+7-N1(7-nitroindazole)+SNL group(n=10 each),with sham group as control(n=10). After propofol intervention, Von Frey method was applied to detect mechanical hypersensitivity in rats. The hot claw foot analgesic device was applied to detect the latent period of the heat contraction foot reflex. Enzyme-linked immunosorbent assay(ELISA) method was applied to detect the expression level of interleukin-6(IL-6) and interferon-γ(IFN-γ) in the spinal cord of rats. RT-qPCR method was used to detect the mRNA expressions of suppressor of cytokine signaling 3(SOCS3), arginase-1(Arg-1) and cluster of differentiation 68(CD68) in the spinal dorsal horn. Western blot was applied to detect the expression level of proteins related to the nitric oxide/cyclic guanosine monophosphate (NO/cGMP) signaling pathway. Results Compared with the sham group, the mechanical foot contraction reflex threshold, thermal foot contraction reflex latency, and the expression level of Arg-1 mRNA, endothelial nitric oxide synthase(eNOS) and soluble guanylate cyclase(sGCa) proteins were significantly reduced in the SNL group(P＜0.05), the level of IL-6 and IFN-γ and the expression level of SOCS3, CD68 mRNA and inducible nitric oxide synthase(iNOS) protein were significantly increased(P＜0.05). Compared with the SNL group, the mechanical foot reflex threshold, thermal foot reflex latency of rats in the propofol+SNL group, the expression level of Arg-1 mRNA, eNOS and sGCa protein were significantly increased(P＜0.05), the level of IL-6 and IFN-γ and the expression of SOCS3, CD68 mRNA and iNOS protein were all decreased(P＜0.05). The NO/cGMP signaling pathway inhibitor 7-N1 reversed the improvement effect of propofol on SNL rats(P＜0.05). Conclusions Propofol alleviates NP and injury in SNL rats by activating the NO/cGMP signaling pathway.

Key words: propofol, spinal nerve ligation, neuropathic pain, NO/cGMP signaling pathway

中图分类号:

R332

高彦东, 边步荣, 王波. 异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛[J]. 基础医学与临床, 2025, 45(8): 1028-1033.

GAO Yandong, BIAN Burong, WANG Bo. Propofol alleviates neuropathic pain induced by spinal nerve ligation in rats[J]. Basic & Clinical Medicine, 2025, 45(8): 1028-1033.

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异丙酚减轻结扎脊神经诱导的大鼠神经病理性疼痛

Propofol alleviates neuropathic pain induced by spinal nerve ligation in rats

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