关键词: 松萝酸, 程序性细胞死亡1(PD-1), 卵巢癌, 增殖, 凋亡

Abstract: Objective To investigate the impacts of usnea acid (UA) on the proliferation of human ovarian cancer (OC) cells. Methods Ovarian adenocarcinoma cells SKOV3 were randomly divided into control group, L-UA group, M-UA group, H-UA group (with 10, 20, and 50 μmol/L UA added respectively), pcDNA3.1-NC group and pcDNA3.1-PD-1 group. RT-qPCR method was applied to detect the expression of programmed cell death 1(PD-1) and programmed cell death ligand 1(PD-L1) in SKOV3 cells. CCK-8 assay and plate method were applied to detect the effect of UA on SKOV3 cell proliferation. The effect of UA on SKOV3 cells apoptosis was examined by flow cytometry. Mouse ovarian epithelial cancer cell line ID8 cells were collected to construct an OC mouse model, and the mass and volume of OC tumors were recorded. Immuno-histochemical microscopy was applied to detect the infiltration of PD-1, PD-L1, and CD8⁺T cells. Results The PD-1 mRNA, PD-L1 mRNA, colony count, A₄₅₀ value, PCNA protein, PD-1 protein, and PD-L1 protein in the L-UA, M-UA, and H-UA groups were lower than those in the control group, the apoptosis rate and Bax protein were higher in the control group (P＜0.05). Compared with the H-UA group and the pcDNA3.1-NC group, the PD-1 mRNA, PD-L1 mRNA, colony count, A₄₅₀ value, PCNA protein, PD-1 protein, and PD-L1 protein in the pcDNA-3.1-PD-1 group increased and the apoptosis rate and Bax protein decreased (P＜0.05). The quality, volume, positivity rate of PD-1and PD-L1 of OC tumors in the UA group was lower than that in the control group, the counting number of CD8⁺T cell infiltration was higher than control group (P＜0.05). Conclusions UA may inhibit progression of OC cell line SKOV3 by regulating cell proliferation, apoptosis, and immune escape.

Key words: usnea acid, programmed cell death 1(PD-1), ovarian cancer, proliferation, apoptosis