CST4在哈萨克族食管鳞状细胞癌中的表达及意义

doi:10.16352/j.issn.1001-6325.2023.09.1412

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (9): 1412-1416.doi: 10.16352/j.issn.1001-6325.2023.09.1412

CST4在哈萨克族食管鳞状细胞癌中的表达及意义

伊尔夏提·图尔宏¹, 张海平¹, 卡吾力·居买¹, 李子垚¹, 张力为¹, 伊地力斯·阿吾提^1,2*

1.新疆医科大学第一附属医院胸外科,新疆乌鲁木齐 830017;
2.新疆医科大学省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐 830017

收稿日期:2022-06-29 修回日期:2022-12-09 出版日期:2023-09-05 发布日期:2023-09-01
通讯作者: ^*docedrs@163.com
基金资助:
国家自然科学基金(81960498);省部共建国家重点实验室开放课题(SKL-HIDCA-2021-21)

Expression and significance of CST4 in Kazakh esophageal squamous cell carcinoma

Yierxiati·TUERHONG¹, ZHANG Haiping¹, Kawuli·JUMAI¹, LI Ziyao¹, ZHANG Liwei¹, Yidilisi·AWUTI^1,2*

1. Department of Thoracic Surgery, the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830017;
2. State Key Laboratory of Pathogenesis, Prevention, Treatment of High Incidence Diseases in Central Asia,Xinjiang Medical University, Urumqi 830017, China

Received:2022-06-29 Revised:2022-12-09 Online:2023-09-05 Published:2023-09-01
Contact: ^*docedrs@163.com

摘要/Abstract

摘要： 目的探究半胱氨酸蛋白酶抑制剂S(CST4)在哈萨克族食管鳞状细胞癌(ESCC)中的表达情况及临床意义。方法收集2010年至2020年于新疆医科大学第一附属医院49例接受手术治疗的哈萨克族食管鳞状细胞癌患者的新鲜组织标本及石蜡包埋的组织标本。用RT-qPCR及免疫组化法检测食管癌组织与对应癌旁组织中CST4的表达。用χ²检验分析CST4表达与患者临床病理资料之间的关系。用单因素及多因素分析CST4表达与患者术后生存期的关系。结果 CST4 mRNA在食管癌组织中的表达量明显高于对应癌旁组织(P＜0.05)。CST4蛋白在食管癌组织中的表达率(53.1%, 26/49)明显高于对应癌旁组织(28.2%, 11/39)(χ²=5.506,P＜0.05)。CST4表达与淋巴结转移(P＜0.05)和TNM分期(P＜0.01)有关,CST4高表达者淋巴结转移率更高,肿瘤TNM分期更晚。单因素生存分析发现,CST4表达水平(P＜0.01)、肿瘤浸润深度(P＜0.05)和TNM分期(P＜0.01)均与患者生存率相关。进一步行多因素生存分析发现,CST4表达水平(P＜0.05)、肿瘤浸润深度(P＜0.05)是影响哈萨克族食管鳞状细胞癌患者预后的独立危险因素。结论 CST4基因的表达可能与哈萨克族食管鳞癌的发生、进展及预后有关。CST4的表达可作为食管鳞癌患者病情及预后评估的指标。

关键词: 食管鳞状细胞癌, 哈萨克族, 半胱氨酸蛋白酶抑制剂S, RT-qPCR, 免疫组织化学

Abstract: Objective To explore the expression and clinical significance of cystatin S(CST4) in patients with esophageal squamous cell carcinoma(ESCC)in Kazakhs. Methods Totally 49 specimens from Kazakh ESCC patients who received surgical treatment were collected. RT-qPCR and immunohistochemical methods were used to detect CST4 expression in cancer tissues and adjacent tissues. Chi-square(χ²) test was used to analyze the relationshipbetween CST4 expression and the clinicopathological parameters. Kaplan-Meier method and Cox regression model were used to estimate the correlation between CST4 expression and overall survival. Results CST4 mRNA expression in ESCC tissues was higher than that in adjacent tissues (P＜0.05). CST4 protein expression in ESCC tissues (53.1%, 26/49) was higher than that in adjacent tissues (28.2%, 11/39) (χ²=5.506, P＜0.05). The expression of CST4 was significantly associated with lymph node metastasis (P＜0.05) and TNM stage (P＜0.01). Those with high CST4 expression had higher lymph node metastasis rate and later TNM stage. Univariate survival analysis showed that CST4 expression level(P＜0.01), invasion depth (P＜0.05) and TNM stage (P＜0.01) were associated with overall survival. Multivariate survival analysis showed that the CST4 expression (P＜0.05) and the invasion depth (P＜0.05) were independent prognostic factors for survival of ESCC patients. Conclusions CST4 expression is related to the occurrence, progression and prognosis of ESCC so it can be a potential prognostic marker for ESCC in Kazakhs.

Key words: esophageal squamous cell carcinoma, Kazakh, cystatin S, RT-qPCR, immunohistochemistry

中图分类号:

R735.1

伊尔夏提·图尔宏, 张海平, 卡吾力·居买, 李子垚, 张力为, 伊地力斯·阿吾提. CST4在哈萨克族食管鳞状细胞癌中的表达及意义[J]. 基础医学与临床, 2023, 43(9): 1412-1416.

Yierxiati·TUERHONG, ZHANG Haiping, Kawuli·JUMAI, LI Ziyao, ZHANG Liwei, Yidilisi·AWUTI. Expression and significance of CST4 in Kazakh esophageal squamous cell carcinoma[J]. Basic & Clinical Medicine, 2023, 43(9): 1412-1416.

参考文献 12

[1]	Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71:209-249.
[2]	左婷婷, 郑荣寿, 曾红梅, 等. 中国食管癌发病状况与趋势分析[J]. 中华肿瘤杂志, 2016, 38:703-708.
[3]	居来提·艾尼瓦尔, 李德生, 张力为, 等. 新疆2005～2008年伊犁地区食管癌患病情况调查研究[J]. 新疆医学, 2011, 41:112-114.
[4]	Dickinson DP, Zhao Y, Thiesse M, et al. Direct mapping of seven genes encoding human type 2 cystatins to a single site located at 20p11.2.[J]. Genomics, 1994, 24:172-175.
[5]	Singhania A, Wallington JC, Smith CG, et al. Multitissue transcriptomics delineates the diversity of airway T cell functions in asthma[J]. Am J Respir Cell Mol Biol, 2018, 58:261-270.
[6]	Martini D, Gallo A, Vella S, et al. Cystatin S-a candidate biomarker for severity of submandibular gland involvement in Sjögren′s syndrome[J]. Rheumatology (Oxford), 2017, 56:1031-1038.
[7]	Dou Y, Lv Y, Zhou X, et al. Antibody-sandwich ELISA analysis of a novel blood biomarker of CST4 in gastrointestinal cancers[J]. Onco Targets Ther, 2018, 11:1743-1756.
[8]	Wang S, Wang C, Liu O, et al. Prognostic value of immune-related cells and genes in the tumor microenviron-ment of ovarian cancer, especially CST4[J]. Life Sci, 2021, 277:119461. doi: 10.1016/j.lfs.2021.119461.
[9]	Johnstone CN, Pattison AD, Gorringe KL, et al. Func-tional and genomic characterisation of a xenograft model system for the study of metastasis in triple-negative breast cancer[J]. Dis Model Mech, 2018, 11: dmm032250. doi: 10.1242/dmm.032250.
[10]	Blanco MA, LeRoy G, Khan Z, et al. Global secretome analysis identifies novel mediators of bone metastasis[J]. Cell Res, 2012, 22:1339-1355.
[11]	Vasiljeva O, Turk B. Dual contrasting roles of cysteine cathepsins in cancer progression: apoptosis versus tumour invasion[J]. Biochimie, 2008, 90:380-386.
[12]	Strojan P, Aničin A, Svetic B, et al. Proteolytic profile of cysteine proteases and inhibitors determines tumor cell phenotype in squamous cell carcinoma of the head and neck[J]. Int J Biol Markers, 2011,26:247-254.

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CST4在哈萨克族食管鳞状细胞癌中的表达及意义

Expression and significance of CST4 in Kazakh esophageal squamous cell carcinoma

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