关键词: 杜仲, 肠壁, 微循环, 血流灌注, 自发性高血压大鼠

Abstract: Objective To investigate the effects of Quanduzhong Jiaonang(QDZJN) and pinoresinol diglucoside (PDG) on microcirculatory blood perfusion in intestinal wall and its spectral characteristics in spontaneously hypertensive rats (SHRs). Methods SHRs were randomly divided into SHR, SHR+QDZJN, SHR+PDG (L), SHR+PDG (M) and SHR+PDG (H) groups with 6 in each group. The control group consisted of 6 WKY rats. All treatment groups were given intragastric administration of QDZJN, PDG, or pure water. After 2 month continuous administration, rats' intestines were exposed by surgery. The wall microcirculatory blood perfusion of was measured by laser Doppler flowmetry (LDF) and laser Doppler perfusion imaging (LDPI). Wavelet transform analysis was performed, and the amplitudes of NO-independent endothelial and NO-dependent endothelial oscillators were compared among six groups. Results Compared with the control group, blood perfusion level of intestine wall was significantly decreased in SHRs group (P＜0.001). After two months of treatment with QDZJN and PDG, the blood perfusion level of SHRs was significantly elevated (P＜0.001). The amplitudes of NO-dependent and -independent endothelial activity in SHRs group were significantly lower than those in the control group (both P＜0.01). QDZJN and PDG significantly enhanced the amplitude of NO-dependent and -independent endothelial activity of intestine wall perfusion in SHRs (P＜0.0001 and P＜0.01). Conclusions Treatment with QDZJN and PDG for two months may significantly improve the microhemodynamics and endothelial function of intestine wall in SHRs.

Key words: Eucommia ulmoides Oliv., intestinal wall, microcirculation, blood perfusion, spontaneously hypertensive rats