miR-216a对人外周血单个核细胞衍生的巨噬细胞特性的影响

doi:10.16352/j.issn.1001-6325.2022.03.034

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 384-388.doi: 10.16352/j.issn.1001-6325.2022.03.034

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miR-216a对人外周血单个核细胞衍生的巨噬细胞特性的影响

韩爽, 陈宇, 张伟丽^*

中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室, 北京 100037

收稿日期:2021-12-30 修回日期:2022-01-12 出版日期:2022-03-05 发布日期:2022-03-04
通讯作者: ^* zhang-weili1747@yahoo.com

Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells

HAN Shuang, CHEN Yu, ZHANG Wei-li^*

State Key Laboratory of Cardiovascular Diseases,National Center for Cardiovascular Diseases and Fuwai Hospital, CAMS & PUMC, Beijing 100037,China

Received:2021-12-30 Revised:2022-01-12 Online:2022-03-05 Published:2022-03-04
Contact: ^* zhang-weili1747@yahoo.com

摘要/Abstract

摘要： 目的探究miR-216a对人外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)来源的巨噬细胞特性的影响。方法分离并培养人PBMCs,诱导建立M1和M2型巨噬细胞极化模型,采用流式细胞术和实时荧光定量PCR方法检测巨噬细胞的表面标记物。进一步,将miR-216a模拟物转染至M2型巨噬细胞,检测M2型巨噬细胞特异标记物及炎性因子基因的表达。结果过表达miR-216a后,M2型巨噬细胞表面标记物CD163的表达水平显著增加,促炎细胞因子TNF-α和IL-1β的表达水平降低,而抗炎细胞因子TGF-β和IL-10的表达水平显著升高(P＜0.05)。结论 miR-216a可促进人PBMCs衍生的巨噬细胞向M2型极化并抑制炎性细胞因子表达。

关键词: miR-216a, 外周血单个核细胞, 巨噬细胞极化, M1型巨噬细胞, M2型巨噬细胞

Abstract: Objective To study the role of miR-216a in regulating the characteristics of macrophages derived from peripheral blood mononuclear cells (PBMCs). Methods Human PBMCs were isolated and cultured. The polarization model of M1 and M2 macrophages were established in vitro, and flow cytometry and real-time quantitative PCR were used to detect the cell surface markers of macrophage. Furthermore, miR-216a mimics were transfected into M2 macrophages, and the surface markers of M2 macrophage and inflammatory factors were examined. Results After overexpression of miR-216a, the expression of surface marker CD163 of M2 macrophage was significantly increased, the expressions of inflammatory cytokines TNFα and IL-1β were decreased, and the expressions of anti-inflammatory cytokines TGF-β and IL-10 were increased(P＜0.05). Conclusions miR-216a could promote the polarization of human PBMCs-derived macrophages into M2 phenotype and inhibit the expressions of pro-inflammatory cytokines.

Key words: miR-216a, peripheral blood mononuclear cell, macrophage polarization, M1 macrophage, M2 macrophage

中图分类号:

R364

韩爽, 陈宇, 张伟丽. miR-216a对人外周血单个核细胞衍生的巨噬细胞特性的影响[J]. 基础医学与临床, 2022, 42(3): 384-388.

HAN Shuang, CHEN Yu, ZHANG Wei-li. Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells[J]. Basic & Clinical Medicine, 2022, 42(3): 384-388.

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miR-216a对人外周血单个核细胞衍生的巨噬细胞特性的影响

Effect of miR-216a on macrophages derived from human peripheral blood mononuclear cells

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