自发性高血压大鼠年龄相关性左心室肥厚的动态变化

doi:10.16352/j.issn.1001-6325.2022.10.1481

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1481-1486.doi: 10.16352/j.issn.1001-6325.2022.10.1481

• 研究论文 • 下一篇

自发性高血压大鼠年龄相关性左心室肥厚的动态变化

易达松^1#, 袁善斌^3#, 曾俊义^1,2*, 赵健清¹, 丁露², 文通¹, 聂俊刚¹

南昌大学第一附属医院 1.心血管内科; 2.江西省高血压病研究所,江西南昌 330006;
3.江西省信丰县人民医院重症医学科,江西信丰 341600

收稿日期:2021-05-24 修回日期:2022-07-18 出版日期:2022-10-05 发布日期:2022-09-23
通讯作者: * zjy1312@163.com
作者简介:^#对本文有相同贡献
基金资助:
国家自然科学基金(81760082);江西省自然科学基金(20181BAB205005);江西省教育厅科学技术研究重点项目(GJJ170040);江西省卫生健康委员会科技计划(202130196)

Dynamic changes of age-related left ventricular hypertrophy found in spontaneously hypertensive rats

YI Da-song^1#, YUAN Shan-bin^3#, ZENG Jun-yi^1,2*, ZHAO Jian-qing¹, DING Lu², WEN Tong¹, NIE Jun-gang¹

1. Department of Cardiology; 2. Jiangxi Hypertension Research Institute,the First Affiliated Hospital of Nanchang University,Nanchang 330006;
3. Department of Critical Care Medicine, People's Hospital of Xinfeng County,Xinfeng 341600, China

Received:2021-05-24 Revised:2022-07-18 Online:2022-10-05 Published:2022-09-23
Contact: * zjy1312@163.com

摘要/Abstract

摘要： 目的观测不同年龄自发性高血压大鼠(SHR)左室肥厚的动态改变。方法选择SPF级4周龄雄性京都种Wistar大鼠(WKY)35只及SHR 45只,连续饲养观测至42周,分别于6、8、12、18、24、32和42周超声心动图监测心脏结构与功能,尾套法监测收缩压,测定左室质量指数(LVMI),HE染色观察左室组织病理形态,RT-qPCR检测左室组织肥厚相关基因ANP和β-MHC。结果 6至12周SHR收缩压快速升高,12周时较WKY大鼠显著升高(P＜0.05),随后SHR血压呈小幅缓慢上升。伴随年龄及血压改变,SHR左室肥厚逐步形成并持续发展,相关肥厚指标室间隔厚度(IVST)、左室后壁厚度(LVPWT)、心肌细胞横径(TDM)及LVMI均呈现特征性动态变化。SHR肥厚相关基因ANP和β-MHC表达也呈年龄及血压依赖性动态改变。结论 SHR左室肥厚伴随年龄及血压改变呈特征性动态变化。

关键词: 自发性高血压大鼠, 左室肥厚, 年龄, 动态变化

Abstract: Objective To study the dynamic changes of left ventricular hypertrophy with age found in spontaneously hypertensive rats (SHR). Methods Forty-five male SHR and thirty-five male Wistar Kyoto rats (WKY), which were four-week-old and specific-pathogen free, were observed continuously for 42 weeks. At 6,8,12,18,24,32 and 42 weeks of age, cardiac structure and function were examined by echocardiography, and systolic blood pressure was measured by tail cuff method; At the same time points, left ventricular mass index (LVMI) was calculated. The morphology of left ventricle was microscopied by HE staining, genes ANP and β-MHC were detected by RT-qPCR. Results The systolic blood pressure of SHR increased rapidly during the period from 6th to 12th weeks, and was significantly higher than that of WKY at 12 weeks (P＜0.05), after that the blood pressure of SHR increased slightly and slowly. With age and blood pressure changes, left ventricular hypertrophy gradually developed in SHR.The related hypertrophic indexes,interventricular septal thickness(IVST), left ventricular posterior wall thickness(LVPWT),transverse diameter of myocardial cell (TDM) and LVMI all showed characteristic changes. Correspondingly, the expression of hypertrophy-related genes ANP and β-MHC in SHR showed age- and blood pressure-dependent dynamic changes. Conclusions The left ventricular hypertrophy in SHR is characterized by the dynamic changes with aging and change of blood pressure.

Key words: spontaneously hypertensive rats, left ventricular hypertrophy, age, dynamic changes

中图分类号:

R544.1

易达松, 袁善斌, 曾俊义, 赵健清, 丁露, 文通, 聂俊刚. 自发性高血压大鼠年龄相关性左心室肥厚的动态变化[J]. 基础医学与临床, 2022, 42(10): 1481-1486.

YI Da-song, YUAN Shan-bin, ZENG Jun-yi, ZHAO Jian-qing, DING Lu, WEN Tong, NIE Jun-gang. Dynamic changes of age-related left ventricular hypertrophy found in spontaneously hypertensive rats[J]. Basic & Clinical Medicine, 2022, 42(10): 1481-1486.

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自发性高血压大鼠年龄相关性左心室肥厚的动态变化

Dynamic changes of age-related left ventricular hypertrophy found in spontaneously hypertensive rats

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