基础医学与临床 ›› 2020, Vol. 40 ›› Issue (8): 1047-1052.

• 研究论文 • 上一篇    下一篇

三胸家族核心成员DPY30与ASH2L的表达相关性分析

文畅1, 李梁1, 舒鹏程1, 强伯勤1, 彭小忠1,2*   

  1. 1.中国医学科学院基础医学研究所 北京协和医学院基础学院 生物化学与分子生物学系医学分子生物学国家重点实验室 医学灵长类研究中心 神经科学中心, 北京 100005;
    2.中国医学科学院 医学生物学研究所, 云南 昆明 650118
  • 收稿日期:2020-05-17 修回日期:2020-06-18 出版日期:2020-08-05 发布日期:2020-07-29
  • 通讯作者: *pengxiaozhong@pumc.edu.cn
  • 基金资助:
    国家自然科学基金 (31970772,31670789)

Analysis of the expression correlation trithorax group protein core components DPY30 and ASH2L

WEN Chang1, LI Liang1, SHU Peng-cheng1, QIANG Bo-qin1, PENG Xiao-zhong1,2*   

  1. 1. State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Medical Primate Research Center, Neuroscience Center, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
    2. Institute of Medical Biology CAMS, Kunming 650118, China
  • Received:2020-05-17 Revised:2020-06-18 Online:2020-08-05 Published:2020-07-29
  • Contact: *pengxiaozhong@pumc.edu.cn

摘要: 目的 探究三胸家族(TrxG)核心成员DPY30与ASH2L表达是否具有相关性。方法 Northern blot检测DPY30与ASH2L在多种人体组织中的表达及佛波醇肉豆蔻酸酯(PMA)诱导人慢性髓源白血病(K562)细胞分化过程中的DPY30与ASH2L表达变化;Western blot与RT-qPCR分别检测ASH2L条件性敲除小鼠(ASH2L-cKO)大脑皮质TrxG成员的蛋白及TrxG核心成员RNA 表达情况;利用IGV软件可视化分析小鼠大脑皮质ChIP-seq结果中ASH2L、H3K4me3在ASH2L、DPY30基因染色质上的分布情况。结果 DPY30与ASH2L的表达在人体各组织中分布有一定相似性;DPY30与ASH2L在PMA诱导K562细胞分化过程中的表达均逐渐减少;ASH2L-cKO小鼠大脑皮质中DPY30蛋白水平减少,DPY30的RNA水平改变无统计学意义。结论 DPY30与ASH2L的表达具有相关性,ASH2L可能调控了DPY30蛋白水平。

关键词: DPY30, ASH2L, 基因表达

Abstract: Objective To investigate the relevance of the expression of DPY30 and ASH2L, two core members of trithorax group proteins (TrxG). Methods Northern blot was used to detect the expression of ASH2L and DPY30 in various human tissues and the trend of ASH2L and DPY30 expression during the differentiation of human chronic myelogenous leukemia (K562) cells induced by PMA. Western blot and RT-qPCR were used to detect the expression of protein and RNA of TrxG core components in the cerebral cortex of ASH2L conditional knockout mouse; IGV software was used to visually analyze the distribution of ASH2L and H3K4me3 on ASH2L and DPY30 gene chromatin in ChIP-seq results of mouse cerebral cortex. Results The expression of DPY30 and ASH2L similar in various tissues of the human body; The expression of DPY30 and ASH2L was both gradually decreasedPMA-induced differ- entiation of K562 cells; DPY30 protein level decreased in the cerebral cortex of ASH2L-cKO mouse while the expression change of DNA level of DPY30 has no statistically significant. Conclusions DPY30 has a correlation with ASH2L expression and ASH2L may regulate the protein expression level of DPY30.

Key words: DPY30, ASH2L, gene expression

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