基础医学与临床 ›› 2018, Vol. 38 ›› Issue (5): 632-637.

• 研究论文 • 上一篇    下一篇

160例非人源细胞系的种间交叉污染情况分析

卞晓翠1,杨振丽2,左春霞1,周芳颖1,刘玉琴2   

  1. 1. 中国医学科学院基础医学研究所;北京协和医学院基础学院
    2. 中国医学科学院基础医学研究所 北京协和医学院基础学院
  • 收稿日期:2017-10-11 修回日期:2017-12-10 出版日期:2018-05-05 发布日期:2018-04-28
  • 通讯作者: 刘玉琴 E-mail:liuyuqin@pumc.edu.cn
  • 基金资助:
    国家科技基础条件平台项目

Analysis of Inter-species cross-contamination in 160 non-human cell lines

  • Received:2017-10-11 Revised:2017-12-10 Online:2018-05-05 Published:2018-04-28
  • Contact: Yu-qin Liu E-mail:liuyuqin@pumc.edu.cn

摘要: 目的 分析常见的非人源细胞系种间交叉污染情况。方法 收集160种常见的非人源细胞系,提取基因组DNA,通过PCR法鉴定其来源种属,判断是否存在种属间的交叉污染。对可疑细胞进一步通过染色体分析确认其来源种属。 结果 160例非人源细胞中,有6个细胞系的种属鉴定结果与预期种属不符,其中1例大鼠来源细胞混入了人来源细胞,其余5例种属不正确,认定为错误细胞。结论 细胞系的种属鉴定是细胞质量控制的不可或缺的环节,只有经过完整质控的细胞才能作为实验材料用于科学研究和应用。

关键词: 非人源细胞系, 细胞培养, 质量控制, 种属鉴定

Abstract: Objective To analyze interspecies cross-contamination of 160 non-human cell lines. Methods One hundred and sixty common non-human cell lines were collected and their species were identified by PCR. For the suspicious cells,chromosome analysis was further used to confirm their species. Results Six in 160 non-human cell lines were cross-contaminated. One rat cell line was mixed by a human cell line, and 5 were totally cross-contaminated, belonging species wrong. Conclusion 3.75% of non-human cell lines were cross-contaminated. Species identification is an indispensable part of cell quality control. Each cell line should undergo a full QA (Quality Assurance) assessment before it is used for research.

Key words: Cell lines, non-human, cell culture, quality control, species identification

中图分类号: