基础医学与临床 ›› 2018, Vol. 38 ›› Issue (3): 350-354.

• 研究论文 • 上一篇    下一篇

RCAN1/CaNA在颞叶癫痫患者及戊四氮动物模型中的表达变化

温跃桃,吴昆仑,谢锐,石全红   

  1. 重庆医科大学附属第一医院
  • 收稿日期:2016-11-04 修回日期:2017-04-24 出版日期:2018-03-05 发布日期:2018-02-27
  • 通讯作者: 石全红 E-mail:417149803@qq.com
  • 基金资助:
    BOLD-fMRI及DTI在功能区脑肿瘤中临床应用的研究

Altered expression of RCAN1/CaNA in temporal lobe epilepsy patients and pentylenetetrazol-induced rats model

  • Received:2016-11-04 Revised:2017-04-24 Online:2018-03-05 Published:2018-02-27

摘要: 目的 观察调神经磷酸酶调节因子1(regulator of calcineurin 1, RCAN1)及钙调神经磷酸酶A(calcineurin A, CaNA)在颞叶癫痫患者及戊四氮(pentylenetetrazol, PTZ)致癫动物模型中的表达变化。方法 收集颞叶癫痫患者手术切除的皮质,并纳入癫痫组(n=18);收集脑外伤患者手术切除的皮质,并纳入对照组(n=11)。30只SD大鼠随机分为模型对照组(model control, MC)和PTZ组。分别应用Western blot和免疫组织化学染色检测RCAN1和CaNA的表达情况;应用免疫荧光染色对RCAN1进行定位;应用免疫共沉淀分析RCAN1与CaNA的相互作用。结果RCAN1定位于神经元的细胞膜;在颞叶癫痫患者及癫痫模型中,RCAN1的表达水平较对照组明显下降(P<0.01),CaNA的表达水平较对照组明显增高(P<0.01);RCAN1与CaNA具有相互作用。结论 在颞叶癫痫患者及癫痫模型中,RCAN1低表达,而CaNA高表达,提示RCAN1可能通过调控CaNA参与癫痫的形成。

关键词: 钙调神经磷酸酶调节因子1, 钙调神经磷酸酶A, 颞叶癫痫, 磷酸化

Abstract: Objective To investigate the expression of regulator of calcineurin 1 (RCAN1) and calcineurin A (CaNA) in brain tissue of temporal lobe (TLE) epilepsy patients and pentylenetetrazol (PTZ)-induced rat model. Methods Cortical samples from 18 patients with temporal lobe epilepsy were collected as epilepsy group and cortical samples from 11 patients with brain trauma were used as control group. 30 SD rats were randomly divided into model control group (MC) and PTZ group. The expression of RCAN1 and CaNA in human brain cortex, rat cortex and rat hippocampus were detected by Western blot and Immunohistochemistry assay. The location analysis of RCAN1 was detected by immunofluorescence. Moreover, co-immunoprecipitation was used to test whether there was an interaction between RCAN1 and CaNA. Results RCAN1 mainly located in neuronal cytomembrane. The expression of RCAN1 was down-regulated and expression of CaNA was up-regulated both in the epileptic patients and epileptic rats (P<0.01). RCAN1 interacted with CaNA. Conclusions Decreased expression of RCAN1 and increased expression of CaNA indicate that RCAN1 may be involved in the epileptogenesis by regulating CaNA.

Key words: regulators of calcineurin 1, calcineurin A, temporal lobe epilepsy, phosphorylation

中图分类号: