miR-207在大鼠纤维化肾组织及尿液中表达上调

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (3): 308-311.

miR-207在大鼠纤维化肾组织及尿液中表达上调

石变华¹,孙佳增²,张尚³,史娟³

1. 中国医学科学院& 北京协和医学院基础医学研究所
2. 中国医学科学院北京协和医学院基础学院
3. 中国医学科学院基础医学研究所

收稿日期:2017-11-17 修回日期:2017-12-19 出版日期:2018-03-05 发布日期:2018-02-27
通讯作者: 史娟 E-mail:shijuantt@yahoo.com
基金资助:
国家重点基础研究发展计划

Expression of miR-207 was up-regulated in renal and urine of rats with renal fibrosis

Received:2017-11-17 Revised:2017-12-19 Online:2018-03-05 Published:2018-02-27

摘要/Abstract

摘要： 目的研究miR-207在大鼠肾脏纤维化(RF)模型中的差异表达，并对其功能进行研究。方法实验组大鼠(UUO)结扎右侧输尿管，假手术组只分离右侧输尿管，不结扎。Real-time PCR定量分析大鼠肾脏纤维化模型肾脏和尿液中miR-207的表达, 以及瞬时转染miR-207 mimics和inhibitors的大鼠肾小管上皮（NRK-52E）细胞中纤维化相关基因的表达。结果大鼠肾脏纤维化模型尿液和肾脏组织中miR-207表达上调（P<0.05），miR-207能上调大鼠肾小管上皮NRK-52E细胞中纤维化相关基因的表达。结论 miR-207可能在肾脏纤维化中发挥重要的作用，为肾脏纤维化诊断靶点提供新的可能。

关键词: 肾脏纤维化, miR-207, NRK-52E

Abstract: Objective To investigate the differential expression of miR-207 in urine samples and kidneys of rat renal fibrosis (renal fibrosis, RF) model and its function was investigated. Methods The rats were divided into two groups, including one undergoing ligation of the right ureter as UUO rat model, the other just under the same surgery but not ligation the ureters as shamed rat model. Real-time PCR (RT-qPCR) was used to detect the expression of miR-207 in kidney and urine of rat renal fibrosis model. The function of miR-207 was investigated with transfection of miR-207 mimics and inhibitor to the rat renal tubular epithelial cells NRK-52E. Results miR-207 expression in urine and kidney tissues of rat renal fibrosis model was upregulated（P<0.05）. miR-207 increased the expression of gene related to fibrosis in rats renal tubular epithelial NRK-52E cells. Conclusions miR-207 might play an important role in renal fibrosis and provide a potential possibility for the diagnosis of renal fibrosis.

Key words: renal fibrosis, miR-207, NRK-52E

中图分类号:

石变华孙佳增张尚史娟. miR-207在大鼠纤维化肾组织及尿液中表达上调[J]. 基础医学与临床, 2018, 38(3): 308-311.